Protein A/G Spin Columns (0.2 mL) (VS-0824-WK13) (CAT#: VS-0824-WK13)
This protein A/G spin column is convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Protein A/G agarose binds nearly all isotypes and mammalian species of IgG from serum, ascites fluid, cell culture supernantant and other antibody samples, thereby enabling effective antibody purification. Because the immobilized Protein A/G combines the immunoglobulin-binding domains of both Protein A and Protein G, the resin is effective for affinity purification of IgG antibodies from a broad range mammalian host species.
Specifications
- Product Size
- 0.2 mL resin/column
- Format
- Microcentrifuge spin column, 10 columns
- Contents
- Each column contains a 0.2 mL resin bed of crosslinked 6% beaded agarose in 0.02 % sodium azide.
- Specifications
- Column type: affinity resin
Support: crosslinked 6 % beaded agarose
Immobilized phase: Protein A/G
Purification target: IgG antibodies
Binding capacity: ≥ 1.4 mg human IgG per column
- Storage
- Upon receipt store columns at 4°C.
- Background
- Protein A/G is a genetically-engineered protein that combines the IgG binding domains of both Protein A and Protein G. The fusion protein is expressed in E. coli. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, resulting in a final mass of 50,460 daltons. Protein A/G is not as pH-dependent as Protein A alone, but otherwise has the additive properties of Protein A and G.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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