Protein A/G Spin Columns (1 mL) (VS-0824-WK14) (CAT#: VS-0824-WK14)
This protein A/G spin column is convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Protein A/G
is a recombinant fusion protein that includes the IgG-binding domains of both Protein A and Protein G. Therefore, Protein A/G is ideal for binding the broadest range of IgG subclasses from rabbit, mouse, human and other mammalian samples. Each column containing 1mL of the immobilized protein resin enables quick purification of ≥ 7mg human IgG. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.
Specifications
- Product Size
- 1 mL resin/column
- Format
- 15 mL conical spin column, 5 columns
- Contents
- Each column contains a 1 mL resin bed of crosslinked 6 % beaded agarose in 0.02 % sodium azide.
- Specifications
- Column type: affinity resin
Support: crosslinked 6 % beaded agarose
Immobilized phase: Protein A/G
Purification target: IgG antibodies
Binding capacity: ≥7 mg human IgG per column
- Storage
- Upon receipt store columns at 4°C.
- Background
- Protein A/G is a genetically-engineered protein that combines the IgG binding domains of both Protein A and Protein G. The fusion protein is expressed in E. coli. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, resulting in a final mass of 50,460 daltons. Protein A/G is not as pH-dependent as Protein A alone, but otherwise has the additive properties of Protein A and G.
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For Research Use Only. Not For Clinical Use.
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