Protein A/G Spin Columns (1 mL) (VS-0824-WK14)
CAT#: VS-0824-WK14
This protein A/G spin column is convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Protein A/G
is a recombinant fusion protein that includes the IgG-binding domains of both Protein A and Protein G. Therefore, Protein A/G is ideal for binding the broadest range of IgG subclasses from rabbit, mouse, human and other mammalian samples. Each column containing 1mL of the immobilized protein resin enables quick purification of ≥ 7mg human IgG. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.
Specifications
- Product Size
- 1 mL resin/column
- Format
- 15 mL conical spin column, 5 columns
- Contents
- Each column contains a 1 mL resin bed of crosslinked 6 % beaded agarose in 0.02 % sodium azide.
- Specifications
- Column type: affinity resin
Support: crosslinked 6 % beaded agarose
Immobilized phase: Protein A/G
Purification target: IgG antibodies
Binding capacity: ≥7 mg human IgG per column
- Storage
- Upon receipt store columns at 4°C.
- Background
- Protein A/G is a genetically-engineered protein that combines the IgG binding domains of both Protein A and Protein G. The fusion protein is expressed in E. coli. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, resulting in a final mass of 50,460 daltons. Protein A/G is not as pH-dependent as Protein A alone, but otherwise has the additive properties of Protein A and G.
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Broad Species Compatibility
These Protein A/G columns are the most versatile purification tool in our fridge. We use them for purifying antibodies from various species, including mouse and rabbit, and the binding capacity is consistently high regardless of the source, which simplifies our workflow.
Efficient For Mixed Isotypes
When we are unsure of the exact isotype or have a mix, these columns are our go-to solution. The combination of Protein A and Protein G binding sites ensures that we capture almost all IgG subclasses efficiently, maximizing our recovery from valuable samples.
Q&As
-
What is Protein A/G and why is it used in these columns?
A: Protein A/G is a recombinant fusion protein that combines the IgG-binding domains of both Protein A and Protein G. This results in a ligand with a broader binding specificity than either protein alone, making these columns capable of binding a wider range of IgG subclasses from various species.
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Are these columns suitable for purifying a mixture of antibodies?
A: Yes, due to their broad binding capability, Protein A/G columns are excellent for purifying mixed samples or when the exact antibody isotype is unknown. They provide a "safety net" ensuring that most IgG variants present in the sample are captured efficiently.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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