Protein A/G Spin Columns (1 mL) (VS-0824-WK14) (CAT#: VS-0824-WK14)
This protein A/G spin column is convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Protein A/G
is a recombinant fusion protein that includes the IgG-binding domains of both Protein A and Protein G. Therefore, Protein A/G is ideal for binding the broadest range of IgG subclasses from rabbit, mouse, human and other mammalian samples. Each column containing 1mL of the immobilized protein resin enables quick purification of ≥ 7mg human IgG. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.
Specifications
- Product Size
- 1 mL resin/column
- Format
- 15 mL conical spin column, 5 columns
- Contents
- Each column contains a 1 mL resin bed of crosslinked 6 % beaded agarose in 0.02 % sodium azide.
- Specifications
- Column type: affinity resin
Support: crosslinked 6 % beaded agarose
Immobilized phase: Protein A/G
Purification target: IgG antibodies
Binding capacity: ≥7 mg human IgG per column
- Storage
- Upon receipt store columns at 4°C.
- Background
- Protein A/G is a genetically-engineered protein that combines the IgG binding domains of both Protein A and Protein G. The fusion protein is expressed in E. coli. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, resulting in a final mass of 50,460 daltons. Protein A/G is not as pH-dependent as Protein A alone, but otherwise has the additive properties of Protein A and G.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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