Protein A/G Spin Kit (0.2 mL) (VS-0824-WK16)
CAT#: VS-0824-WK16
This protein A/G spin kit is convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilized protein resin enables quick purification of ≥ 1.4mg human IgG from serum or other sample. The actual amount of IgG purified varies depending on the sample type and the specific spin column used. Also included in each kit are sufficient collection tubes, buffers and a streamlined protocol for purifying at least 10 antibody samples.
Specifications
- Product Size
- 0.2 mL resin/column
- Format
- Microcentrifuge spin column, 10 columns kit
- Contents
- Spin columns, 10 columns; each column contains a 0.2 mL resin bed of crosslinked 6 % beaded agarose in 0.02 % sodium azide
Microcentrifuge tubes, 2 mL, 80 tubes
Binding buffer, 1 pack (100 mM phosphate, 150 mM sodium chloride; pH 7.2 when dissolved in 500 mL of ultrapure water)
IgG elution buffer, 50 mL, pH 2.8
Neutralization buffer, 12 mL, 1 M Tris•HCl, pH 8.5
- Specifications
- Column type: affinity resin
Support: crosslinked 6 % beaded agarose
Immobilized phase: Protein A/G
Purification target: IgG antibodies
Binding capacity: ≥ 1.4 mg human IgG per column
- Storage
- Upon receipt store kit at 4°C.
- Background
- Protein A/G is a genetically-engineered protein that combines the IgG binding domains of both Protein A and Protein G. The fusion protein is expressed in E. coli. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, resulting in a final mass of 50,460 daltons. Protein A/G is not as pH-dependent as Protein A alone, but otherwise has the additive properties of Protein A and G.
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