Protein A IgG Binding Buffer (VS-0824-WK25)
CAT#: VS-0824-WK25
This protein A IgG binding buffer is a phosphate-based buffer containing glycerol and EDTA, formulated for antibody purification using Protein A.
Specifications
- Product Size
- 1 L
- Format
- Liquid
- Contents
- pH 8.0; phosphate-based; contains glycerol and EDTA
- Specifications
- Reagent type: binding buffer
pH: 8
- Storage
- Store at 4°C.
- Background
- This buffer has a long shelf life and is pre-mixed for maximum ease of use.
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Optimizes Binding Efficiency
This buffer significantly improved the binding of our rabbit IgG to Protein A columns. We used to make our own buffers, but switching to this optimized solution resulted in less antibody in the flow-through and a higher overall yield in the final elution.
Reliable And Consistent
We process many antibody samples weekly, and consistency is key. This binding buffer has been reliable across different lots, ensuring that our purification process is standardized and reproducible, which is critical for our downstream antibody characterization.
Q&As
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How does this binding buffer improve antibody purification?
A: This buffer is optimized to provide the ideal pH and ionic strength for the interaction between Protein A and IgG. This maximization of binding efficiency ensures that more antibody is captured by the column and less is lost in the flow-through, ultimately leading to higher yields.
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Is this buffer suitable for use with Protein G columns as well?
A: While it is specifically optimized for Protein A, the binding conditions for Protein A and G can overlap. However, for best results with Protein G, we recommend checking the specific requirements of your resin or using a universal binding buffer if available. This product is best used for Protein A affinity chromatography.
View the frequently asked questions answered by Creative Biolabs Support.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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