Protein A Spin Columns (1 mL) (VS-0824-WK2) (CAT#: VS-0824-WK2)
This Protein A spin column is convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each column containing 1 mL of the immobilized protein resin enables quick purification of ≥34 mg of human IgG from 0.5-2 mL of serum or other samples. Typically, the immobilized protein column may be used up to 10 times without significant loss in binding capacity.
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Specifications
- Product Size
- 1 mL resin/column
- Format
- 15 mL conical spin column, 5 columns
- Contents
- Each column contains a 1 mL resin bed of crosslinked 6% beaded agarose in 0.02% sodium azide.
- Specifications
- Column type: affinity resin
Support: crosslinked 6 % beaded agarose
Immobilized phase: Protein A
Purification target: IgG antibodies
Binding capacity: ≥34 mg human IgG per column or 16-17 mg mouse IgG/mL resin
- Storage
- Upon receipt store columns at 4°C.
- Background
- Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7 kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A contains four high-affinity binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6). Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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