Protein A Spin Columns (5 mL) (VS-0824-WK3)
CAT#: VS-0824-WK3
This Protein A spin column is convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each column containing 5 mL of the immobilized protein resin enables quick purification of ≥175 mg human IgG or 80-85 mg mouse IgG per column from serum or other samples. Typically, the immobilized protein column may be used up to 10 times without significant loss in binding capacity.
Specifications
- Product Size
- 5 mL resin/column
- Format
- 50 mL conical spin column, 1 column
- Contents
- Each column contains a 5 mL resin bed of crosslinked 6% beaded agarose in 0.02% sodium azide.
- Specifications
- Column type: affinity resin
Support: crosslinked 6 % beaded agarose
Immobilized phase: Protein A
Purification target: IgG antibodies
Binding capacity: ≥175 mg human IgG or 80-85 mg mouse IgG per column
- Storage
- Upon receipt store columns at 4°C.
- Background
- Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7 kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A contains four high-affinity binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6). Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.
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