Protein A Spin Kit (0.2 mL) (VS-0824-WK4) (CAT#: VS-0824-WK4)
This protein A spin kit is convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilized protein resin enables quick purification of 100-1000μg of IgG from 25-500μL of serum or other sample. The actual amount of IgG purified varies depending on the sample type and the specific spin column used. Also included in each kit are sufficient collection tubes, buffers and a streamlined protocol for purifying at least 10 antibody samples.
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Specifications
- Product Size
- 0.2 mL resin/column
- Format
- Microcentrifuge spin column, 10 columns kit
- Contents
- Spin columns, 10 columns; each column contains a 0.2 mL resin bed of crosslinked 6 % beaded agarose in 0.02 % sodium azide
Microcentrifuge tubes, 2 mL, 80 tubes
Binding buffer, 1 pack (100mM phosphate, 150mM sodium chloride; pH 7.2 when dissolved in 500mL of ultrapure water)
IgG elution buffer, 50 mL, pH 2.8
Neutralization buffer, 12 mL, 1 M Tris•HCl, pH 8.5
- Specifications
- Column type: affinity resin
Support: crosslinked 6 % beaded agarose
Immobilized phase: Protein A
Purification target: IgG antibodies
Binding capacity: ≥7 mg human IgG per column
- Storage
- Upon receipt store kit at 4°C.
- Background
- Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7 kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A contains four high-affinity binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6). Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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