Protein G Spin Columns (1 mL) (VS-0824-WK8)
CAT#: VS-0824-WK8
This protein G spin column is convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each column containing 1 mL of the immobilized protein resin enables quick purification of 11-15mg human IgG per column. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.
Specifications
- Product Size
- 1 mL resin/column
- Format
- 15 mL conical spin column, 5 columns
- Contents
- Each column contains a 1 mL resin bed of crosslinked 6 % beaded agarose in 0.02 % sodium azide.
- Specifications
- Column type: affinity resin
Support: crosslinked 6 % beaded agarose
Immobilized phase: Protein G
Purification target: IgG antibodies
Binding capacity: 11-15 mg human IgG per column
- Storage
- Upon receipt store columns at 4°C.
- Background
- Protein G is a bacterial cell wall protein original from group G Streptococcus and now produced as a recombinant in E. coli. Like Protein A, Protein G binds to most mammalian immunoglobulins primarily through their Fc regions. Native Protein G contains two immunoglobulin binding sites, as well as albumin and cell surface binding sites. In the recombinant form of Protein G, these albumin and cell surface binding sites have been eliminated to reduce nonspecific binding when purifying immunoglobulins. Recombinant Protein G has a mass of approximately 21.6kDa, but its apparent size by SDS-PAGE is 31 to 34kDa. IgG-binding function is optimal at pH 5 but also occurs efficiently in near-neutral conditions (pH 7.0 to 7.2). Protein G Agarose is effective for affinity purification of IgG from serum and other fluids of many mammalian species. Protein G is especially well suited for use with mouse antibodies (including IgG1) in addition to most IgG isotypes from human, goat and sheep samples.
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Fast Spin Column Format
These spin columns are incredibly convenient for small-scale purifications. I was able to purify multiple mouse IgG1 samples in parallel within a short time frame, avoiding the hassle of setting up peristaltic pumps and tubing for traditional chromatography.
Great For Mouse Antibodies
Since Protein A binds poorly to some mouse sub-isotypes, these Protein G columns were exactly what we needed. They bound our mouse monoclonal antibodies very efficiently, and the purity of the eluted fraction was high enough for our immediate use in cell assays.
Q&As
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What is the advantage of the spin column format?
A: The spin column format offers speed and convenience. It eliminates the need for complex liquid chromatography systems (like pumps and fraction collectors). You can perform the binding, washing, and elution steps using a standard benchtop centrifuge, allowing for the parallel processing of multiple samples.
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When should I choose Protein G columns over Protein A?
A: Protein G is generally preferred when purifying antibodies that have weak affinity for Protein A, such as mouse IgG1 and human IgG3. If you are working with mouse monoclonals or a variety of species like goat or sheep, Protein G spin columns are often the better choice for higher recovery.
View the frequently asked questions answered by Creative Biolabs Support.
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