Protein L Spin Kit (1 mL) (VS-0824-WK22) (CAT#: VS-0824-WK22)
This protein L spin kit is convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilized protein resin enables quick purification of 5-10 mg of human IgG from serum or other sample. Included in each kit are buffers and a streamlined protocol for purifying at least two antibody samples.
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Specifications
- Product Size
- 1 mL resin/column
- Format
- 15 mL conical spin column, 2 columns kit
- Contents
- Spin columns, 2 columns; each column contains a 1 mL resin bed of crosslinked 6 % beaded agarose in 0.02 % sodium azide
Binding buffer, 1 pack (100 mM phosphate, 150 mM sodium chloride; pH 7.2 when dissolved in 500 mL of ultrapure water)
IgG elution buffer, 240 mL, pH 2.8
Neutralization buffer, 12 mL, 1 M Tris•HCl, pH 8.5
- Specifications
- Column type: affinity resin
Support: crosslinked 6 % beaded agarose
Immobilized phase: Protein L
Purification target: IgG antibodies
Binding capacity: 5-10 mg human IgG per column
- Storage
- Upon receipt store kit at 4°C.
- Background
- Protein L binds to certain immunoglobulin kappa light chains. Because kappa light chains occur in members of all classes of immunoglobulin (that is, IgG, IgM, IgA, IgE and IgD), Protein L can purify these different classes of antibody. However, only those antibodies within each class that possess the appropriate kappa light chains will bind. Generally, empirical testing is required to determine if Protein L is effective for purifying a particular antibody of interest.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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