Recombinant Protein A Agarose (VS-0824-WK6)
CAT#: VS-0824-WK6
This recombinant Protein A agarose is a genetically engineered form of Protein A that is produced from a nonpathogenic strain of Bacillus. Although some non-essential regions have been removed, the recombinant is similar to native Protein A in size and IgG-binding sites. The protein has four high affinity binding sites capable of specifically binding to the Fc region of immunoglobulin molecules from several species. Protein A is heat-stable and retains its native conformation even when exposed to denaturing reagents such as 4M urea, 4M thiourea and 6M guanidine hydrochloride. This Recombinant Protein A Agaroseis prepared using a coupling method that results in excellent resin stability and binding characteristics. The special Protein A buffers enhance IgG binding, resulting in higher yields compared with conventional methods.
Specifications
- Product Size
- 5 mL
- Format
- Bottle
- Contents
- 5 mL settled agarose resin
- Specifications
- Support: crosslinked 6 % beaded agarose supplied as a 50 % slurry containing 0.02 % sodium azide
Immobilized phase: Protein A
Purification target: IgG antibodies
Binding capacity: 6-8mg mouse or 15-17mg human polyclonal IgG per mL of settled resin using Protein A IgG purification buffers
- Storage
- Upon receipt store product at 4°C to 8°C.
- Background
- Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7 kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A contains four high-affinity binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6). Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.
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