Antibody Structural Characterization Service
Diving Deep into Antibody Architecture for Therapeutic Assurance
Understanding the intricate structural properties of antibody molecules is a critical component of biologic drug development. Structural characterization provides detailed insights into an antibody's molecular architecture, including its conformational stability, folding patterns, disulfide connectivity, glycosylation profiles, and aggregation status. These attributes directly influence a therapeutic antibody's efficacy, pharmacokinetics, safety, and manufacturability. At Creative Biolabs, we offer a dedicated antibody structural characterization service to deliver high-resolution structural insights essential for the development, optimization, and regulatory submission of therapeutic antibodies.
Fig.1 Different antibody formats.
Mapping the Molecular Blueprint: Our Service Scope
We offer: Our structural characterization platform provides multi-tiered analytical solutions tailored to comprehensively evaluate the architecture of monoclonal antibodies, bispecific antibodies, antibody fragments, and other Ig-based formats. Our service capabilities include but are not limited to:
- Primary Structure Confirmation: Accurate mass measurement and peptide mapping to verify sequence integrity and detect potential truncations or substitutions.
- Secondary and Tertiary Structure Evaluation: Spectroscopic and calorimetric approaches to assess folding, stability, and conformational changes.
- Disulfide Bridge Mapping: Identification of native and scrambled disulfide bonds critical to correct folding.
- Glycosylation Pattern Analysis: In-depth profiling of glycan structures, site occupancy, and microheterogeneity.
- Aggregation and Fragmentation Assessment: Quantification and characterization of high molecular weight species and degradation products.
- Structural Comparability Studies: Evaluation of biosimilarity at the structural level for follow-on biologics and process changes.
Each analytical pathway is designed based on the specific needs of the molecule and the development phase, ensuring that data output is relevant, reproducible, and actionable.
Powered by Precision: Core Technologies Behind Our Platform
Our antibody structural studies are supported by a suite of state-of-the-art technologies, enabling highly sensitive and specific analysis of each structural layer:
- LC-MS/MS & HR-MS: For intact mass analysis, peptide mapping, and disulfide bond determination with exceptional accuracy.
- Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS): A powerful technique for investigating protein folding, domain dynamics, and epitope mapping.
- Circular Dichroism (CD) and Fourier Transform Infrared Spectroscopy (FTIR): Essential for assessing secondary structure content and conformational stability.
- Differential Scanning Calorimetry (DSC): Measurement of thermal unfolding to determine protein stability profiles.
- Size-Exclusion Chromatography (SEC) with MALS or UV Detection: Quantitative analysis of aggregates, fragments, and monomer purity.
- Capillary Electrophoresis (CE-SDS): Assessment of intact and reduced antibody chains under denaturing conditions.
These technologies are applied in a modular or integrated manner depending on the characterization objectives, allowing a highly flexible and scientifically rigorous approach.
From Inquiry to Insight: Our Structured Workflow
Creative Biolabs implements a streamlined and customizable workflow to ensure transparency, efficiency, and data accuracy throughout the structural characterization process:
This approach allows us to adapt rapidly to evolving client needs while maintaining the scientific depth required for high-stakes biologic programs.
Built for Biologics: Distinct Advantages of Our Structural Analysis
Our structural characterization service offers a combination of scientific acumen, technological capability, and client-centric flexibility that sets us apart:
Specialized Focus on Antibodies
Years of experience with diverse antibody formats, including Fc-engineered, glycoengineered, and non-canonical structures.
Orthogonal Validation
We employ multiple complementary techniques to validate findings and reduce analytical blind spots.
Customization at Every Step
Whether for early research or other filing, we scale our services to fit your specific goals and timelines.
Rapid Turnaround, Reliable Results
Efficient project coordination ensures timely delivery without compromising scientific accuracy.
A Trusted Scientific Partner for Your Structural Analysis
Creative Biolabs is more than a service provider—we are a dedicated partner in your antibody development journey. By combining technical precision with collaborative engagement, we help de-risk the structural characterization process and empower data-driven decision-making. Our team of protein chemists, structural analysts, and regulatory consultants works in unison to ensure that you receive meaningful insights, not just data.
Partner with us to unlock the structural secrets of your antibody candidates. Contact our team today and discover how we can support your program with structural clarity and scientific confidence.
For research use only. Not intended for any clinical use.
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