10-FnIII scaffold protein anti-ySUMO (CAT#: SFN-L031)
The tenth fibronectin type III domain (10Fn3) that bound ySUMO was isolated from the library using Phage display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity (Kd=5 nM) maturation, 10Fn3 variants were identified having affinities for ySUMO as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
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Specifications
- Scaffold Name
- 10-FnIII
- Origin
- Fibronectin
- Core Structure
- β-sandwich of 7 β-strands
- Variable Regions
- 2–3 Loops
- Target
- ySUMO
- Species Reactivity
- yeast
- Expression Host
- BL21 (DE3) cells
- Affinity Constant
- 5 nM
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
- Alternative Names
- Fibronectin; 10-FnIII; IDDM5; SMT3H4; SUMO-4; dJ281H8.4
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-3463 | Hi-Affi™ Recombinant Rabbit Anti-SUMO4 Monoclonal Antibody (DS3463AB) | WB, ICC, IP, FC | IgG |
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