APC-H-2Db/Influenza A virus NP (ASNENMDAM) MHC Tetramer
CAT#: MHC-LC539
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of Influenza-derived peptide of ASNENMDAM sequence covering 366-374 and H-2Db molecule. The pMHC tetramer recognizes mouse CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.
Specifications
- Allele
- H-2Db
- Class
- Class I
- MHC Species
- Mouse
- Antigen
- Influenza A virus NP
- Antigen Species
- Influenza A virus
- Peptide
- ASNENMDAM
- Range
- 366-374
- Conjugate
- APC
- Application
- FCM
Target
- Antigen Introduction
- Influenza A virus causes influenza in birds and some mammals, and is the only species of influenza virus A. Influenza virus A is a genus of the Orthomyxoviridae family of viruses. Strains of all subtypes of influenza A virus have been isolated from wild birds, although disease is uncommon. Some isolates of influenza A virus cause severe disease both in domestic poultry and, rarely, in humans. Occasionally, viruses are transmitted from wild aquatic birds to domestic poultry, and this may cause an outbreak or give rise to human influenza pandemics.
- Alternative Names
- Influenza A virus NP; Influenza A virus Nucleoprotein
- Gene ID
- 956531
- UniProt ID
- P03466
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Fantastic binding efficiency
Using this MHC tetramer in our flow cytometry setup greatly increased our capacity to quantify Influenza-specific T cells. The specificity and binding efficiency are outstanding.
Applicable to immune kinetics post-infection
We're impressed with the performance of this tetramer. It allowed us to follow antigen-specific T cell responses over multiple time points, giving us crucial insights into immune kinetics post-infection.
High-quality product
High-quality product! The tetramer provides clear and distinct staining in our T cell assays. This reagent has made our studies on Influenza-specific immunity so much more efficient.
Q&As
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To what extent may antigen-specific T cells be identified using the APC-H-2Db/Influenza A virus NP (ASNENMDAM) MHC Tetramer?
A: This tetramer is a useful tool for immunological research and vaccine development since it has high specificity and sensitivity in identifying T cells that recognize the Influenza A virus NP epitope.
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Is the Tetramer appropriate for use in applications involving flow cytometry?
A: Indeed, allophycocyanin (APC) conjugation makes this MHC tetramer perfect for use in flow cytometry studies to accurately define T cells that are specific to antigens.
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Does the MHC Tetramer for APC-H-2Db/Influenza A virus NP call for any particular staining procedures?
A: The typical tetramer staining procedure in flow cytometry would be sufficient. It is recommended that the cells be incubated with the tetramer for around 30 minutes at room temperature prior to analysis.
View the frequently asked questions answered by Creative Biolabs Support.
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