Recombinant Human Anti-C. botulinum botA Antibody (3D12) (CAT#: EPAF-0045LC)
This product is a human monoclonal antibody that specifically recognizes Botulinum neurotoxin type A precursor from C. botulinum. The botA-binding antibody 3D12 is an epitope-specific antibody that can be used in ELISA and functional studies.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
SDS-PAGE
Figure 1 Recombinant Human Anti-C. botulinum botA Antibody (3D12) (EPAF-0045LC) in SDS-PAGE
SDS-PAGE analysis of EPAF-0045LC in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
WB
Figure 2 Recombinant Human Anti-C. botulinum botA Antibody (3D12) (EPAF-0045LC) in WB
Western blot analysis of EPAF-0045LC was performed by loading Recombinant Clostridium botulinum Botulinum neurotoxin type A(botA) (expressed in E. coli, his Tag).
EPAF-0045LC incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
Specifications
- Host Species
- Human
- Type
- IgG
- Specificity
- Clostridium botulinum Botulinum neurotoxin type A precursor
- Species Reactivity
- C. botulinum
- Clone
- 3D12
- Applications
- Enzyme-linked Immunosorbent Assay, Functional Assay
- Related Disease
- Flaccid paralytic
Target
- Alternative Names
- C. botulinum botA; Clostridium botulinum Botulinum neurotoxin type A precursor; C. botulinum; Clostridium botulinum; Botulinum neurotoxin type A precursor; botA
- UniProt ID
- P10845
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Epitope-Specific Antibodies
Single Domain Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0042LC | Recombinant Mouse Anti-C. botulinum botA Antibody (HuC25) | FC | IgG |
| EPAF-0043LC | Recombinant Mouse Anti-C. botulinum botA Antibody (S25) | FC | IgG |
| EPAF-0044LC | Recombinant Mouse Anti-C. botulinum botA Antibody (C25) | ELISA, FuncS | IgG |
| EPAF-0106CQ | Recombinant Mouse Anti-C. botulinum botA Antibody (1C6) | ELISA | IgG |
| EPAF-0107CQ | Recombinant Mouse Anti-C. botulinum botA Antibody (1F3) | ELISA | IgG |
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