Recombinant Human Anti-C. botulinum botA Antibody (3D12) (CAT#: EPAF-0045LC)
This product is a human monoclonal antibody that specifically recognizes Botulinum neurotoxin type A precursor from C. botulinum. The botA-binding antibody 3D12 is an epitope-specific antibody that can be used in ELISA and functional studies.
Specific Inquiry
SDS-PAGE
Figure 1 Recombinant Human Anti-C. botulinum botA Antibody (3D12) (EPAF-0045LC) in SDS-PAGE
SDS-PAGE analysis of EPAF-0045LC in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
WB
Figure 2 Recombinant Human Anti-C. botulinum botA Antibody (3D12) (EPAF-0045LC) in WB
Western blot analysis of EPAF-0045LC was performed by loading Recombinant Clostridium botulinum Botulinum neurotoxin type A(botA) (expressed in E. coli, his Tag).
EPAF-0045LC incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
Specifications
- Host Species
- Human
- Type
- IgG
- Specificity
- Clostridium botulinum Botulinum neurotoxin type A precursor
- Species Reactivity
- C. botulinum
- Clone
- 3D12
- Applications
- Enzyme-linked Immunosorbent Assay, Functional Assay
- Related Disease
- Flaccid paralytic
Target
- Alternative Names
- C. botulinum botA; Clostridium botulinum Botulinum neurotoxin type A precursor; C. botulinum; Clostridium botulinum; Botulinum neurotoxin type A precursor; botA
- UniProt ID
- P10845
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0042LC | Recombinant Mouse Anti-C. botulinum botA Antibody (HuC25) | FC | IgG |
| EPAF-0043LC | Recombinant Mouse Anti-C. botulinum botA Antibody (S25) | FC | IgG |
| EPAF-0044LC | Recombinant Mouse Anti-C. botulinum botA Antibody (C25) | ELISA, FuncS | IgG |
| EPAF-0106CQ | Recombinant Mouse Anti-C. botulinum botA Antibody (1C6) | ELISA | IgG |
| EPAF-0107CQ | Recombinant Mouse Anti-C. botulinum botA Antibody (1F3) | ELISA | IgG |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-579 | Recombinant Alpaca Anti-C. botulinum botA Single Domain Antibody (PABC-579) | Neut | Alpaca VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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