Anti-CGB immunotoxin PiPP (scFv)-PE (CAT#: AGTO-G012E)
This immunotoxin was achieved by conjugating/fusing the Anti-CGB scFv to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to human chorionic gonadotropin (hCG) expressing cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating T-lymphoblastic leukemia and lung carcinoma.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38925/537_C10_3_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38925/537_C10_3_selected.jpg
Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38925/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38925/ihc_selected.jpg
Figure 3 Placenta
(Trophoblastic cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38934/101364_A_2_7.jpg
(Trophoblastic cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38934/101364_A_2_7.jpg
Figure 4 RNA cell line category: Cell line enriched (BEWO)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104827-CGB3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104827-CGB3
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- PiPP
- Molecule Class
- scFv-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- T-lymphoblastic leukemia and lung carcinoma
Target
- Alternative Names
- CGB; chorionic gonadotropin, beta polypeptide; CGB3; CGB5; CGB7; CGB8; hCGB; choriogonadotropin subunit beta; CG-beta; chorionic gonadotropin beta chain; chorionic gonadotrophin chain beta; chorionic gonadotropin beta subunit; chorionic gonadotropin beta 3 subunit; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE38
- Gene ID
- 1082
- UniProt ID
- P01233
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1405z | Mouse Anti-CGB Recombinant Antibody (clone 30H6) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| PABW-134 | Mouse Anti-CGB Recombinant Antibody (clone 3468) | ELISA, WB | Mouse IgG |
| MOB-0046MZ | Recombinant Mouse Anti-Human hCG beta Antibody (clone A057-10019) | ELISA | Mouse antibody |
| VS3-CJ243 | Mouse Anti-CGB Recombinant Antibody (VS3-CJ243) | IHC | Mouse IgG |
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