anti-CRH immunotoxin PFU 83 (IgG)-RTA
CAT#: AGTO-L007R
This immunotoxin was achieved by conjugating/fusing the Anti-CRH monoclonal antibody to ricin toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to CRF neurons cells. Then the ricin A chain is incorporated into the cell by receptor-mediated endocytosis, blocking ribosomal protein synthesis and ultimately leading to cell death. It was designed for treatment of Anorexia nervosa.
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000147571-CRH
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Specifications
- Host Species
- Rat
- Species Reactivity
- Human
- Clone
- PFU 83
- Molecule Class
- L kappa; IgG2a-RTA
- Toxin Description
- Ricin consist of two polypeptide chains, A and B, linked by means of disulfide bridge(s). Cytotoxicity is associated with the A chain and its 15 inhibition of protein synthesis in nucleated cells. The B chain is essentially a delivery vehicle for the A chain. The B chain recognizes polysaccharide units at the surface of cells and creates a high affinity interaction with such units. Once the B chain binds with polysaccharide units at 20" the celL surface, the A chain is incorporated into the cell, blocking ribosomal protein synthesis and ultimately leading tocell death. The use of ricin A chain is preferred in this invention.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Anorexia nervosa
Target
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Benchmark for Specificity
This antibody has established a standard for specificity in our laboratory. There are no cross-reactions, and the results are clear-it's ideal for our needs.
User-friendly
The antibody is easy to use and effective in a wide range of applications. Preparation and use are both uncomplicated.
Excellent Support
Customer care was extremely prompt and provided effective answers to all of our technical questions. This type of support is quite beneficial.
Q&As
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Do I need to block non-specific binding sites before using the antibody?
A: Yes, blocking non-specific binding sites is essential. We recommend using a blocking solution like 5% BSA or non-fat milk in PBS for best results.
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The signal is weak; what could be the problem?
A: Weak signal can be due to insufficient antibody concentration, inadequate incubation time, or poor antigen retrieval. Increasing primary antibody concentration or extending incubation times can help.
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Does this antibody show cross-reactivity with other proteins?
A: Our antibody is validated for high specificity and shows minimal cross-reactivity with other proteins, making it ideal for precise experimental outcomes.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Diseases
Alcoholism
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone PFU 83"
- CAT
- Product Name
See other products for "CRH"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2504z | Mouse Anti-CRH Recombinant Antibody (clone 8F1) | WB, ELISA, IP | Mouse IgG2a, κ |
| MOB-1700MZ | Recombinant Mouse Anti-Human CRH Antibody (clone 5I0) | ELISA, IHC-Fr, IHC-P | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0135MZ | Chicken Anti-CRH Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1670 | Anti-CRH Immunohistochemistry Kit | IHC |
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