Recombinant Camel Anti-CST3 Single Domain Antibody (HPAB-0350-YC-VHH)
CAT#: HPAB-0350-YC-VHH
The product is a sdAb aiming at cystatin C (CysC) epitope and the sdAb can be efficiently expressed in escherichia coli to benefit the research on cystatin C(CysC) detection reagents.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13143/97_A12_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Cytosol
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/642_E9_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/2084_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/2084_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/2084_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Proximal tubules (cell body)
Staining:High
Intensity: Strong
Quantity: 75%-25%
Proximal tubules (microvilli)
Staining:High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/2084_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/2084_A_4_6.jpg
Normal Tissue
Figure 9 Epididymis
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/2084_A_3_6.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000101439-CST3
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Specifications
- Host Species
- Camel
- Derivation
- Phage display library
- Type
- Camel VHH
- Specificity
- Human CST3
- Species Reactivity
- Human
- Clone
- HPAB-0350-YC-VHH
- Applications
- ELISA
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
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Customer Review
There are currently no Customer reviews or questions for HPAB-0350-YC-VHH. Click the button above to contact us or submit your feedback about this product.
Excellent for ELISA
I used the Recombinant Camel Anti-CST3 Antibody in an ELISA assay, and the results were outstanding. The specificity and sensitivity were significantly better than other antibodies I have tried.
Stable and Reliable
I've had the same vial for months, and it remains potent. Consistency is key in research, and this antibody delivers.
Great Vendor Support
Not just the product, but the support from the company has been excellent. Quick responses and helpful tips for my experiments.
Q&As
-
Does this antibody work in ELISA?
A: Yes, the Recombinant Camel Anti-CST3 Single Domain Antibody is validated for use in ELISA. We have found it performs exceptionally well in detecting CST3 in various samples.
-
What is the isotype of this antibody?
A: Being a single-domain antibody (VHH), it does not have a conventional isotype but is derived from camelid heavy-chain antibodies.
-
Is cross-reactivity with other cystatins a concern?
A: This antibody is highly specific to CST3 with minimal cross-reactivity with other cystatins according to the provided data.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0350-YC-VHH, RRID: AB_3111391)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "CST3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1014 | Mouse Anti-CST3 Recombinant Antibody (clone YC029); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-048 | Recombinant Anti-Human CST3 VHH Single Domain Antibody | IHC, FC, CA, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0648MZ | Chicken Anti-Human Cystatin C Polyclonal IgY | N/A | Chicken antibody |
| BRD-0649MZ | Chicken Anti-Human Cystatin C complete IgY egg yolk Polyclonal IgY | N/A | Chicken antibody |
| BRD-1127MZ | Chicken Anti-Human Cystatin C Polyclonal IgY | Indirect ELISA | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0844 | Hi-Affi™ Rabbit Anti-CST3 Recombinant Antibody (clone DS844AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0170U | Mouse Anti-CST3 Recombinant Antibody (clone 3A1B7) | ELISA, IHC | Mouse IgG2b |
| VS3-QX288 | Mouse Anti-CST3 Recombinant Antibody (clone 4A9-8D8-1D5) | WB, IHC, ELISA | Mouse IgG1 |
| VS3-QX289 | Mouse Anti-CST3 Recombinant Antibody (clone 6A4-8C9-4B1) | WB, IHC, ELISA | Mouse IgG1 |
| VS3-QX290 | Mouse Anti-CST3 Recombinant Antibody (clone 7E3-4G2-9C8) | WB, IHC, ELISA | Mouse IgG1 |
| VS3-WK1332 | Mouse Anti-CST3 Recombinant Antibody (VS3-WK1332) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC204 | AbPlus™ Anti-Cst3 Magnetic Beads (VS-0724-YC204) | IP, Protein Purification | |
| VS-0724-YC261 | AbPlus™ Anti-CST3 Magnetic Beads (VS-0724-YC261) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1734 | Anti-CST3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1735 | Anti-Rat CST3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1736 | Anti-Mouse CST3 Immunohistochemistry Kit | IHC |
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