Mouse Anti-Collagen type II Recombinant Antibody (clone CIIC1)
CAT#: PABW-028 Autoantibody
Recombinant Mouse Antibody (CIIC1) is capable of binding to Collagen type II, expressed in Chinese Hamster Ovary cells (CHO). Monoclonal anti-collagen type II antibody CIIC1 is an arthritogenic autoantibody, which induces arthritis in mice.
Specifications
- Immunogen
- Mouse Collagen type II
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Mouse Collagen type II
- Species Reactivity
- Mouse
- Clone
- CIIC1
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Collagen type II antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Surface Plasmon Resonance.
Enzyme-linked Immunosorbent Assay: CII (1 μg/well) was coated to 96 Micro Well MaxiSorp™ Enzyme-linked Immunosorbent Assay plates (Nunc, Denmark) overnight at 4°C whereas the wells for blank controls were coated with 4‐μg BSA (bovine serum albumin). After coating, the wells were blocked with 2% BSA in phosphate buffered saline (PBS) for 1 h and subsequently incubated with the CIIC1scFv at different dilutions overnight. The His‐tag served for the detection of bound CIIC1scFv by an unlabeled Penta‐His Antibody (Qiagen), diluted 1:500 in PBS pH 7.4 and incubated for 2 h at room temperature.
SPR: Surface Plasmon Resonance measurements were performed on a BIAcore 2000 instrument. CII (100 μg/mL) diluted in 10 mM acetate buffer, pH 4.5 dissolved in running buffer (HBS‐EP [0.01 M HEPES, pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% Surfactant P20]) was covalently immobilized on the CM5 sensor chip at a density of 600 response units by using standard amine‐coupling chemistry.
Target
- Alternative Names
- Collagen type II
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone CIIC1"
- CAT
- Product Name
See other products for "Collagen type II"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-028 | Mouse Anti-Collagen type II Recombinant Antibody (clone CIIC1); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-028 | Mouse Anti-Collagen type II Recombinant Antibody (clone CIIC1); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1726CQ | Mouse Anti-Collagen type II Recombinant Antibody (clone mAb 1-5) | ELISA | Mouse IgG1 |
| ZG-0184J | Mouse Anti-Collagen Type II Recombinant Antibody (ZG-0184J) | IHC-P, IF | Mouse IgG |
| ZG-0185J | Mouse Anti-Collagen Type II Recombinant Antibody (ZG-0185J) | IHC-P, IF | Mouse IgG |
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