Afuco™ Anti-Human ESR2 ADCC Recombinant Antibody (Mayo MC10), ADCC Enhanced (CAT#: AFC-237CL)

Anti-ESR2 ADCC Enhanced Antibody (Mayo MC10) is an ADCC enhanced antibody produced by our Afuco™ platform. The use of the MC10 antibody, in combination with highly specific antibodies targeting only full-length ERα, is likely to provide additional discriminatory features in breast cancers that may be useful in predicting response to therapy.


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Figure 1 Specificity of MC9 and MC10 monoclonal antibodies for ERβ protein as determined by immunoprecipitation and western blotting.

Figure 1 Specificity of MC9 and MC10 monoclonal antibodies for ERβ protein as determined by immunoprecipitation and western blotting.

293T cells were transfected with either a Flag-tagged ERα (A) or Flag-tagged ERβ (B) expression vector for 24 h and equal amounts of cell lysates were immunoprecipitated with indicated antibodies. Immunoprecipitated proteins (IP) were separated by SDS-PAGE and western blotting (WB) was performed using indicated antibodies. Detection of non-immunoprecipitated ERα or ERβ protein levels were also determined by western blotting in whole cell extracts (WCE).

Wu, X,Subramaniam,M.,Negron,V.,Cicek,M.,Reynolds,C.,Lingle,W.L., & Hawse,J. R. (2012).Development,characterization,and applications of a novel estrogen receptor beta monoclonal antibody. Journal of cellular biochemistry, 113(2),711-723.

Figure 2 Specificity and comparison of the MC10 monoclonal antibody for ERβ protein to that of other commercial antibodies as determined by immunohistochemistry.

Figure 2 Specificity and comparison of the MC10 monoclonal antibody for ERβ protein to that of other commercial antibodies as determined by immunohistochemistry.

U2OS cells expressing either Flag-tagged ERβ or ERα under the control of a doxycycline inducible promoter were pelleted, paraffin embedded, sectioned, and processed for immunohistochemistry. Sections of un-induced cells, ERβ expressing cells and ERα expressing cells were stained with a monoclonal Flag antibody (1:100) (A), an ERα antibody (1: 50) (B), or the following ERβ antibodies (F), and PPG5/10 (1:100) (G).

Wu, X,Subramaniam,M.,Negron,V.,Cicek,M.,Reynolds,C.,Lingle,W.L., & Hawse,J. R. (2012).Development,characterization,and applications of a novel estrogen receptor beta monoclonal antibody. Journal of cellular biochemistry, 113(2),711-723.

Figure 3 Detection of full-length ERβ1 and its variants (ERβ2-5) by the MC10 and PPG5/10 monoclonal antibodies.

Figure 3 Detection of full-length ERβ1 and its variants (ERβ2-5) by the MC10 and PPG5/10 monoclonal antibodies.

293T cells were transiently transfected with expression vectors for ERβ1–5 for 24 h. Equal amounts of whole cell lysates were separated by SDS-PAGE and western blotting (WB) was performed using the PPG5/10 and MC10 antibodies. PPG5/10 was shown to only detect ERβ1 while the MC10 antibody was shown to cross-react with ERβ1-5.

Wu, X,Subramaniam,M.,Negron,V.,Cicek,M.,Reynolds,C.,Lingle,W.L., & Hawse,J. R. (2012).Development,characterization,and applications of a novel estrogen receptor beta monoclonal antibody. Journal of cellular biochemistry, 113(2),711-723.


Specifications

  • Host Species
  • Human
  • Type
  • ADCC enhanced antibody
  • Species Reactivity
  • Human
  • Related Disease
  • Breast Cancer

Product Property

  • Purity
  • >95% by HPLC
  • Storage
  • Store at –20 or -70°C upon receipt. Divide antibody into aliquots prior usage and avoid multiple freeze-thaw cycles.

Target

  • Alternative Names
  • ESR2; estrogen receptor 2 (ER beta); Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA; estrogen receptor beta; estrogen receptor beta 4; nuclear receptor subfamily 3 group A member 2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ESR2"

Rat Antibody

CAT Product Name Application Type
TAB-249CL Human Anti-ESR2 Recombinant Antibody (TAB-249CL) ELISA Human IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1202 Hi-Affi™ Rabbit Anti-ESR2 Recombinant Antibody (clone DS1202AB) ICC, IF, IP, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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