Recombinant Mouse Antibody (Ab53) is capable of binding to F. tularensis GroEL, expressed in Chinese Hamster Ovary cells (CHO).
Figure 1 Mouse Anti-F. tularensis GroEL Antibody (Ab53) in ELISA.
Ab12, Ab53, and Ab64 bind FtGroEL but only Ab12 and Ab53 crossreact with E. coli. ELISA (data from one of two experiments with similar results are shown). Lysates of E. coli BL21 transformed with pET14b vector containing Ft SchuS4 GroEL DNA, Ft LVS GroEL DNA, or no insert (empty vector) were coated on ELISA plates in the left panels, and greater than 10-fold higher concentrations of SchuS4, LVS, or E. coli TG1 lysates were coated on ELISA plates in the right panels. The coated plates were probed with serial dilutions of the indicated mAbs.
Lu, Z., Rynkiewicz, M. J., Madico, G., Li, S., Yang, C. Y., Perkins, H. M., ... & Sharon, J. (2014). B-cell epitopes in GroEL of Francisella tularensis. PloS one, 9(6), e99847.
Figure 2 Mouse Anti-F. tularensis GroEL Antibody (Ab53) in WB.
Ab64 binds to a different FtGroEL epitope than Ab12 and Ab53. Isotype-specific competition WB. LVS lysate nitrocellulose strips were pretreated with either 800 µg/ml of Ab12 (competitor mAb, when indicated), or were mock-treated, and then 5 µg/ml of Ab12, Ab53 or Ab64 (primary mAb) was incubated with the membrane strips. Anti (a)-m (mouse)-IgG (H+L, binds IgG1, IgG2b and IgG3) or a-m-IgG2a-specific alkaline phosphatase (AP) conjugates (second Ab) were used for development, as indicated.
Lu, Z., Rynkiewicz, M. J., Madico, G., Li, S., Yang, C. Y., Perkins, H. M., ... & Sharon, J. (2014). B-cell epitopes in GroEL of Francisella tularensis. PloS one, 9(6), e99847.
Figure 3 Mouse Anti-F. tularensis GroEL Antibody (Ab53) in ELISA.
Only one of 14 FtGroEL mAbs crossreacts with human or mouse Hsp60. ELISA. Human or mouse recombinant Hsp60 was coated on ELISA plates, and the plates were probed with serial dilutions of the indicated mAbs.
Lu, Z., Rynkiewicz, M. J., Madico, G., Li, S., Yang, C. Y., Perkins, H. M., ... & Sharon, J. (2014). B-cell epitopes in GroEL of Francisella tularensis. PloS one, 9(6), e99847.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-420 | Recombinant Anti-GroEL Antibody | WB, IHC, FuncS | IgG |
PABC-228 | Recombinant Mouse Anti-F. tularensis GroEL Antibody (Ab64) | ELISA, WB | IgG |
MRO-069CQ | Recombinant Mouse Anti-C. trachomatis Hsp60 Antibody (A57-B9) | WB, IP, IF | IgG |
MRO-070CQ | Recombinant Mouse Anti-C. trachomatis Hsp60 Antibody (A57-E4) | WB | IgG |
MOB-2443MZ | Recombinant Mouse Anti-Escherichia coli groEL Antibody (clone 0B2/3) | IP, WB | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-420-F(E) | Recombinant Anti-GroEL Antibody Fab Fragment | IP, RIA, FuncS | Fab |
MHH-420-F(E) | Recombinant Human Anti-GroEL Antibody Fab Fragment | WB, IHC, FuncS | Fab |
PSBC-228 | Recombinant Mouse Anti-F. tularensis GroEL Antibody scFv Fragment (Ab64) | ELISA, WB | scFv |
PSBC-229 | Recombinant Mouse Anti-F. tularensis GroEL Antibody scFv Fragment (Ab53) | ELISA, WB | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-420-S(P) | Recombinant Anti-GroEL Antibody scFv Fragment | WB, FC, FuncS | scFv |
MHH-420-S(P) | Recombinant Human Anti-GroEL Antibody scFv Fragment | Neut, WB, FuncS | scFv |
PFBC-228 | Recombinant Mouse Anti-F. tularensis GroEL Antibody Fab Fragment (Ab64) | ELISA, WB | Fab |
PFBC-229 | Recombinant Mouse Anti-F. tularensis GroEL Antibody Fab Fragment (Ab53) | ELISA, WB | Fab |
There are currently no Customer reviews or questions for PABC-229. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.