Recombinant Mouse Anti-F. tularensis GroEL Antibody (Ab53) (CAT#: PABC-229)

Recombinant Mouse Antibody (Ab53) is capable of binding to F. tularensis GroEL, expressed in Chinese Hamster Ovary cells (CHO).


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ELISA

Figure 1 Mouse Anti-F. tularensis GroEL Antibody (Ab53) in ELISA.

Figure 1 Mouse Anti-F. tularensis GroEL Antibody (Ab53) in ELISA.

Ab12, Ab53, and Ab64 bind FtGroEL but only Ab12 and Ab53 crossreact with E. coli. ELISA (data from one of two experiments with similar results are shown). Lysates of E. coli BL21 transformed with pET14b vector containing Ft SchuS4 GroEL DNA, Ft LVS GroEL DNA, or no insert (empty vector) were coated on ELISA plates in the left panels, and greater than 10-fold higher concentrations of SchuS4, LVS, or E. coli TG1 lysates were coated on ELISA plates in the right panels. The coated plates were probed with serial dilutions of the indicated mAbs.

Lu, Z., Rynkiewicz, M. J., Madico, G., Li, S., Yang, C. Y., Perkins, H. M., ... & Sharon, J. (2014). B-cell epitopes in GroEL of Francisella tularensis. PloS one, 9(6), e99847.

WB

Figure 2 Mouse Anti-F. tularensis GroEL Antibody (Ab53) in WB.

Figure 2 Mouse Anti-F. tularensis GroEL Antibody (Ab53) in WB.

Ab64 binds to a different FtGroEL epitope than Ab12 and Ab53. Isotype-specific competition WB. LVS lysate nitrocellulose strips were pretreated with either 800 µg/ml of Ab12 (competitor mAb, when indicated), or were mock-treated, and then 5 µg/ml of Ab12, Ab53 or Ab64 (primary mAb) was incubated with the membrane strips. Anti (a)-m (mouse)-IgG (H+L, binds IgG1, IgG2b and IgG3) or a-m-IgG2a-specific alkaline phosphatase (AP) conjugates (second Ab) were used for development, as indicated.

Lu, Z., Rynkiewicz, M. J., Madico, G., Li, S., Yang, C. Y., Perkins, H. M., ... & Sharon, J. (2014). B-cell epitopes in GroEL of Francisella tularensis. PloS one, 9(6), e99847.

ELISA

Figure 3 Mouse Anti-F. tularensis GroEL Antibody (Ab53) in ELISA.

Figure 3 Mouse Anti-F. tularensis GroEL Antibody (Ab53) in ELISA.

Only one of 14 FtGroEL mAbs crossreacts with human or mouse Hsp60. ELISA. Human or mouse recombinant Hsp60 was coated on ELISA plates, and the plates were probed with serial dilutions of the indicated mAbs.

Lu, Z., Rynkiewicz, M. J., Madico, G., Li, S., Yang, C. Y., Perkins, H. M., ... & Sharon, J. (2014). B-cell epitopes in GroEL of Francisella tularensis. PloS one, 9(6), e99847.


Specifications

  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native F. tularensis GroEL
  • Species Reactivity
  • F. tularensis
  • Clone
  • Ab53
  • Applications
  • Can be useful in applications such as: Enzyme-linked Immunosorbent Assay, Western Blot

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

  • Alternative Names
  • HSPD1; CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13; heat shock protein family D (Hsp60) member 1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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