Recombinant Mouse Anti-F. tularensis O-antigen Antibody (N62) (CAT#: PABL-378)
Recombinant Mouse Antibody (N62) is capable of binding to F. tularensis O-antigen , expressed in Chinese Hamster Ovary cells (CHO).
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Figure 1 Western blot analysis of N62 binding to both long and short lipopolysaccharide (LPS) chains.
Ab63, N213 and N62, like FB11, bind strongly to both long and short lipopolysaccharide (LPS) chains on Western blots. Two hundred microlitres of SchuS4 lysate (equivalent to 0·05 OD600 of original bacterial culture) or Francisella tularensis LPS (20 μg) was electrophoresed in a preparative 4–15% polyacrylamide gel (8·6 cm wide by 6·8 cm long) under denaturing conditions. The positions of molecular weight markers are indicated. Strips were probed with the indicated monoclonal antibodies (mAbs) at the indicated μg/ml concentrations and AP‐conjugated anti‐mouse IgG (H + L) was used as secondary antibody. The terminally binding anti‐OAg mAb FB11 (IgG2a), and the internally binding anti‐OAg mAbs Ab52 (IgG2a) and Ab2 (IgG3) were included for comparison.
Lu, Z., Rynkiewicz, M. J., Yang, C. Y., Madico, G., Perkins, H. M., Wang, Q., ... & Sharon, J. (2013). The binding sites of monoclonal antibodies to the non‐reducing end of F rancisella tularensis O‐antigen accommodate mainly the terminal saccharide. Immunology, 140(3), 374-389.
Specifications
- Immunogen
- Francisella tularensis O-antigen
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native F. tularensis O-antigen
- Species Reactivity
- F. tularensis
- Clone
- N62
- Applications
- WB 1:1000-1:10000
ELISA 1:1000-1:20000
FuncS Assay-depended
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- O-antigen; F. tularensis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-091 | Recombinant Mouse Anti-F. tularensis O-antigen Antibody (PABL-091) | WB, ELISA, FuncS | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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