Mouse Anti-GCN4 Recombinant Antibody (clone C11L34) (CAT#: PABC-049)
Recombinant Mouse Antibody (C11L34) is capable of binding to GCN4(7P-14P), expressed in Chinese Hamster Ovary cells (CHO). This antibody has an affinity of 40 pM.
Specific Inquiry
WB
Figure 1 Immunoblots of cell extracts showing much stronger binding to the tandem epitope with longer linker.
Lysates from HEK cells expressing PCDH15 with different tags were run on two identical gels; one was probed with the divalent C11L34 anti-GCN at a fixed concentration and one with anti-PCDH15 as a loading control.
Zhao, H. , Shen, A. , Xiang, Y. K. , & Corey, D. P.. (2016). Three recombinant engineered antibodies against recombinant tags with high affinity and specificity. PLOS ONE, 11.
Figure 2 Binding of recombinant C11L34 antibody to a single or tandem GCN epitope placed within the PCDH15 sequence and expressed in RPMI 2650 cells.
Binding was normalized to the signal of an anti-PCDH15 antibody. The binding to the tandem epitope was about 15x better, if the linker was 19 amino acids (green line) but not if the linker was shorter (magenta).
Zhao, H. , Shen, A. , Xiang, Y. K. , & Corey, D. P.. (2016). Three recombinant engineered antibodies against recombinant tags with high affinity and specificity. PLOS ONE, 11.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Clone
- C11L34
- Applications
- WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody C11L34 has been reported in applications of Western Blot, Immunoprecipitation, Immunofluorescence, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-389 | Recombinant Anti-GCN4 Antibody | ELISA, WB, FuncS | IgG |
| MHH-389 | Recombinant Human Anti-GCN4 Antibody | FC, RIA, Biosensors, FuncS | IgG |
| FAMAB-1711CQ | Mouse Anti-GCN4 Recombinant Antibody (clone SW1) | ELISA, ITC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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