Human Anti-HNE Recombinant Antibody (clone R310) (CAT#: PABL-197)
Recombinant Human Antibody (R310) is capable of binding to HNE, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HNE mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HNE mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody showed only a slight crossreactivity with the native double-stranded DNA, whereas the Ab immunoreactivity was dramatically enhanced by the treatment of the DNA with 4-oxo-2-nonenal (ONE), an analog of HNE.
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Figure 1 Crossreactivity of anti-R-HNE mAb R310 with the protein-bound HNE enantiomers.
Akagawa, M., Ito, S., Toyoda, K., Ishii, Y., Tatsuda, E., Shibata, T.,... & Adachi, T. (2006). Bispecific abs against modified protein and DNA with oxidized lipids. Proceedings of the National Academy of Sciences, 103(16), 6160-6165.
Figure 2 Anti-DNA affinity of anti-HNE mAbs.
(A) Crossreactivity of the anti-HNE mAbs R310 and S412 with calf-thymus DNA. ○, S412; ●, R310. (B) Crossreactivity of the anti-HNE mAbs and anti-DNA mAb D466, obtained from 56R and CD40L double-transgenic mice, with native DNA and oligonucleoside 50-mers. The Ab reactivity was measured by direct antigen ELISA. (C) Immunoreactivity of R310 and S412 toward aldehyde-treated DNA. In A–C, affinity of the Abs was determined by a direct antigen ELISA using aldehyde-treated DNA as the absorbed antigens. A coating antigen was prepared by incubating calf-thymus DNA (1 mgml) with 5 mM aldehydes in 1 ml of 0.1 M sodium phosphate buffer (pH 7.4) for 24 h at 37°C. One microgram of antigen (calf-thymus DNA or oligonucleoside 50-mers) was coated per well in polystyrene plates, and Ab binding was detected.
Akagawa, M., Ito, S., Toyoda, K., Ishii, Y., Tatsuda, E., Shibata, T.,... & Adachi, T. (2006). Bispecific abs against modified protein and DNA with oxidized lipids. Proceedings of the National Academy of Sciences, 103(16), 6160-6165.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- HNE
- Clone
- R310
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for ELISA. For details, refer to published data.
Target
- Alternative Names
- 4-hydroxy-2-nonenal; 4-HNE; HNE; 4-Hydroxynonenal
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-197 | Human Anti-HNE Recombinant Antibody (clone R310); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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