Human Anti-HNE Recombinant Antibody (clone R310) (CAT#: PABL-197)

Figure 1 Crossreactivity of anti-R-HNE mAb R310 with the protein-bound HNE enantiomers.

Akagawa, M., Ito, S., Toyoda, K., Ishii, Y., Tatsuda, E., Shibata, T.,... & Adachi, T. (2006). Bispecific abs against modified protein and DNA with oxidized lipids. Proceedings of the National Academy of Sciences, 103(16), 6160-6165.

Figure 2 Anti-DNA affinity of anti-HNE mAbs.

(A) Crossreactivity of the anti-HNE mAbs R310 and S412 with calf-thymus DNA. ○, S412; ●, R310. (B) Crossreactivity of the anti-HNE mAbs and anti-DNA mAb D466, obtained from 56R and CD40L double-transgenic mice, with native DNA and oligonucleoside 50-mers. The Ab reactivity was measured by direct antigen ELISA. (C) Immunoreactivity of R310 and S412 toward aldehyde-treated DNA. In A–C, affinity of the Abs was determined by a direct antigen ELISA using aldehyde-treated DNA as the absorbed antigens. A coating antigen was prepared by incubating calf-thymus DNA (1 mgml) with 5 mM aldehydes in 1 ml of 0.1 M sodium phosphate buffer (pH 7.4) for 24 h at 37°C. One microgram of antigen (calf-thymus DNA or oligonucleoside 50-mers) was coated per well in polystyrene plates, and Ab binding was detected.

Akagawa, M., Ito, S., Toyoda, K., Ishii, Y., Tatsuda, E., Shibata, T.,... & Adachi, T. (2006). Bispecific abs against modified protein and DNA with oxidized lipids. Proceedings of the National Academy of Sciences, 103(16), 6160-6165.