Rabbit Anti-HOXA9 Recombinant Antibody (clone DS1658AB)
CAT#: MOR-1658
Recombinant Rabbit anti-HOXA9 monoclonal antibody for WB, FC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61982/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61982/139006_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61982/139006_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61982/139006_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61982/139006_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61982/139006_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61982/139006_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HEK93, PC-3, RPTEC TERT1, SCLC-21H, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078399-HOXA9
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Specifications
- Immunogen
- Synthetic peptide (C terminal).
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human HOXA9
- Species Reactivity
- Human
- Clone
- DS1658AB
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Superior Western Blot Results
The recombinant rabbit monoclonal format of clone DS1658AB delivered exceptionally clean bands in our Western Blot experiments. We were able to clearly identify HOXA9 expression levels in our cell lysates, and the lot-to-lot consistency of the Hi-Affi antibodies is very reassuring for long-term projects.
Great For Flow Cytometry
We utilized this antibody for Flow Cytometry analysis of hematopoietic cells. The specificity for the C-terminal peptide of HOXA9 was evident, as we observed distinct population shifts corresponding to transcription factor expression. The recombinant production really seems to enhance sensitivity.
Q&As
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How was this antibody generated and what is the immunogen?
A: We developed this Hi-Affi™ antibody using phage display library technology, which allows for humane generation without traditional ascitic fluid. The immunogen used is a synthetic peptide corresponding to the C-terminus of Human HOXA9, ensuring specific recognition of the target transcription factor.
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What applications is this antibody validated for?
A: Our recombinant rabbit monoclonal antibody (clone DS1658AB) is validated for use in Western Blot (WB) and Flow Cytometry (FC). It is designed to produce superior results with greater specificity and sensitivity, making it suitable for studying HOXA9 expression in embryonic development or leukemogenesis research.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "HOXA9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2388MZ | Recombinant Mouse Anti-Human HOXA9 Antibody (clone IPY6J054) | ELISA, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX538 | Mouse Anti-HOXA9 Recombinant Antibody (clone 6C-7C) | WB, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-0425-WR78 | Mouse Anti-HOXA9 Recombinant Antibody (clone 1A12C1) | ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-0425-WR79 | Mouse Anti-HOXA9 Recombinant Antibody (clone 5C7C6) | ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3254 | Anti-HOXA9 Immunohistochemistry Kit | IHC |
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