Mouse Anti-HSPA1A Recombinant Antibody (clone cmHsp70.1) (CAT#: HPAB-0243-YC)

Figure 1 Representative views of the kinetics of cmHsp70.1-FITC mAb uptake into CT26 tumour cells.

Representative views of the kinetics of cmHsp70.1-FITC mAb uptake into CT26 tumour cells. Tumour cells were washed and analysed by flow cytometry following incubation with cmHsp70.1-FITC mAb or IgG1-FITC for 2, 5, 10, 15, 30 and 60 min. cells at 4°C (left panel) and 37°C (right panel). The upper graphs indicate the percentage of positively stained cells the lower graphs indicate the antigen densities at the indicated time-points, expressed as the mfi. The increase in the proportion of membrane Hsp70⁺cells and in the mfi was significant (P < 0.05) at 37°C but not at 4°C.

Stangl, S., Gehrmann, M., Dressel, R., Alves, F., Dullin, C., Themelis, G.,... & Multhoff, G. (2011). In vivo imaging of CT26 mouse tumours by using cmHsp70. 1 monoclonal antibody. Journal of cellular and molecular medicine, 15(4), 874-887.

Figure 2 (C) Quantitative analysis of the fluorescence intensity images of the tumours of mice that received either cmHsp70.1-Cy5.5 mAb (black bars) or IgG1-Cy5.5 (white bars).

(C) Quantitative analysis of the fluorescence intensity images of the tumours of mice that received either cmHsp70.1-Cy5.5 mAb (black bars) or IgG1-Cy5.5 (white bars). Average intensities of fluorescence signals in the s.c. tumour regions of the two mice shown in (B) at the indicated time-points 0, 24, 48, 72 and 96 hrs after i.v. injection of the antibodies are displayed. The data were corrected for their labelling intensities. (D) Kinetics of average fluorescence intensity of cmHsp70.1-Cy5.5 mAb (black dots) and IgG1-Cy5.5 control (white dots) in tumour-bearing mice. The data represent a summary of the average fluorescence intensity over tumour regions in mice at 24, 48, 72 and 96 hrs after i.v. injection of the antibodies. Data represent mean values of five animals; * marks values P < 0.05; ** marks values P < 0.001.

Stangl, S., Gehrmann, M., Dressel, R., Alves, F., Dullin, C., Themelis, G.,... & Multhoff, G. (2011). In vivo imaging of CT26 mouse tumours by using cmHsp70. 1 monoclonal antibody. Journal of cellular and molecular medicine, 15(4), 874-887.

Figure 3 Capacity of cmHsp70.1 mAb to induce ADCC against CT26 tumour cells in vitro.

In vitro ADCC of CT26 colon (containing 55% Hsp70 membrane-positive cells) carcinoma cells, using 0.7, 1 and 1.4 μg/ml cmHsp70.1 mAb and unstimulated mouse spleen cells at E:T ratios ranging from 100:1 to 12.5:1. The data show one representative experiment out of three independent experiments showing similar results.

Stangl, S., Gehrmann, M., Dressel, R., Alves, F., Dullin, C., Themelis, G.,... & Multhoff, G. (2011). In vivo imaging of CT26 mouse tumours by using cmHsp70. 1 monoclonal antibody. Journal of cellular and molecular medicine, 15(4), 874-887.

Figure 1 Recombinant Mouse Anti-HSPA1A Antibody (HPAB-0243-YC) in SDS-PAGE

Lane M: Marker
Lane 1: HPAB-0243-YC in non-reduced condition.
Lane 2: HPAB-0243-YC in reduced condition.

Figure 2 Recombinant Mouse Anti-HSPA1A Antibody (HPAB-0243-YC) in HPLC

The purity of HPAB-0243-YC was greater than 92% as determined by SEC-HPLC.