Anti-HTNV G1 Recombinant Antibody (6D4)
CAT#: MRO-1071LC
This product is a recombinant mouse anti-Hantaan Virus monoclonal antibody. 6D4 specifically binds to G1 protein associated with HTNV and can be potentially used in the treatment and diagnosis of Hantaan Virus infection.
Published Data
IP
Figure 1 Expression from pWRG/AND-M.
The indicated HTNV-specific MAbs, HTNV-specific mouse hyperimmune ascitic fluid (HTN-poly), or HPS convalescent-phase serum from Argentina (AND-poly) was used to immunoprecipitate proteins from COS cells transfected with pWRG/HTN-M(x) (H) or pWRG/AND-M (A). +, G1 or G2 was immunoprecipitated; −, no protein was immunoprecipitated; *, both G1 and G2 were immunoprecipitated.
Active and Passive Vaccination against Hantavirus Pulmonary Syndrome with Andes Virus M Genome Segment-Based DNA Vaccine
WB
Figure 2 Transient expression of HTNV G1 and G2.
COS cells were transfected with pWRG/HTN-M or a negative control plasmid (pWRG7077) and, after 24 h, radiolabeled cell lysates were prepared for analysis by RIPA. Expression products were immunoprecipitated with a polyclonal mouse hyperimmune ascitic fluid against HTNV (HTN HMAF), a G1-specific MAb (MAb 6D4), or a G2-specific MAb (MAb 23G10).
DNA Vaccination with the Hantaan Virus M Gene Protects Hamsters against Three of Four HFRS Hantaviruses and Elicits a High-Titer Neutralizing Antibody Response in Rhesus Monkeys
IP
Figure 3 SDS-PAGE of affinity-purified recombinant human IgG antibodies to HTNV.
Radiolabeled HTNV proteins were immunoprecipitated with HFRS patient serum (lane HP) or with recombinant human antibodies as indicated above each lane. The positions of the viral envelope glycoproteins, G1 and G2, and the nucleocapsid protein, N, are indicated to the left of the autoradiogram.
Generation of an HFRS Patient-Derived Neutralizing Recombinant Antibody to Hantaan Virus G1 Protein and Definition of the Neutralizing Domain
ELISA
Figure 4 Binding of recombinant human antibodies AH100IgG (G1- specific) and AH30IgG (N-specific) to sucrose gradient-purified HTNV, strains 76–118 and 84Fli, and SEOV, strain L99 as measured by ELISA.
Values are expressed as % of maximum binding of OD value at 490 nm.
Generation of an HFRS Patient-Derived Neutralizing Recombinant Antibody to Hantaan Virus G1 Protein and Definition of the Neutralizing Domain
IF
Figure 5 Immunofluorescence assay of recombinant human antibody AH100IgG with HTNV-infected Vero-E6 cells or with HTN M fragment specific recombinant baculovirus infected SF9 cells.
FITC conjugated anti-human IgG Fc antibodies were used for the detection. Cell culture supernatants of SF9 cells infected with recombinant baculoviruses expressing human IgG antibodies to G1 and N were incubated with the slides at 378C for 30 min. Bound antibodies were detected with FITC conjugated anti-human human Fc antibodies.
Generation of an HFRS Patient-Derived Neutralizing Recombinant Antibody to Hantaan Virus G1 Protein and Definition of the Neutralizing Domain
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Specifications
- Host Species
- Mouse
- Type
- Mouse Antibody
- Specificity
- Hantaan Virus
- Clone
- 6D4
- Applications
- ELISA, IP, WB, IF
- Related Disease
- Hantaan Virus infection
Applications
- Application Notes
- The HTNV G1 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunoprecipitation, Western Blot, Immunofluorescence.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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See other products for "HTNV G1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1070LC-F(E) | Anti-HTNV G1 Recombinant Antibody Fab Fragment (8B6) | ELISA | Mouse Antibody |
| MRO-1071LC-F(E) | Anti-HTNV G1 Recombinant Antibody Fab Fragment (6D4) | ELISA | Mouse Antibody |
| MRO-1072LC-F(E) | Anti-HTNV G1 Recombinant Antibody Fab Fragment (10F11) | ELISA | Mouse Antibody |
| MRO-1073LC-F(E) | Anti-HTNV G1 Recombinant Antibody Fab Fragment (2D5) | ELISA | Mouse Antibody |
| MRO-1074LC-F(E) | Anti-HTNV G1 Recombinant Antibody Fab Fragment (3D5) | ELISA | Mouse Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY137 | AbPlus™ Anti-HTNV G1 Magnetic Beads (8B6) | IP, Protein Purification |
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