What is the target of Brazikumab?

Brazikumab is a human recombinant monoclonal antibody that targets Human IL23A (Interleukin 23 subunit alpha). It binds to the p19 subunit of IL-23, which is involved in cytokine signaling.

Can this antibody be used to inhibit IL-23 activity?

Yes, this antibody is reported for use in Inhibition (Inhib) assays. It is also suitable for ELISA, Immunohistochemistry (IHC), Flow Cytometry (FC), Immunoprecipitation (IP), and Immunofluorescence (IF).

Anti-Human IL23A Recombinant Antibody (Brazikumab)

CAT#: TAB-008ML

Recombinant monoclonal antibody to IL23A. Brazikumab is a human monoclonal antibody that can be potentially used in the treatment of Crohn's disease (CD).

Tested Data Gene Expression

SDS-PAGE

Figure 1 Anti-Human IL23A Therapeutic Antibody (Brazikumab) (TAB-008ML) in SDS-PAGE

SDS-PAGE analysis of TAB-008ML in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.

SEC-HPLC

Figure 2 Anti-Human IL23A Therapeutic Antibody (Brazikumab) (TAB-008ML) in SEC-HPLC

HPLC analysis of TAB-008ML shows the purity is>95%.

Figure 3 Anti-Human IL23A Therapeutic Antibody (Brazikumab) (TAB-008ML) in WB

Western blot analysis was performed by loading 0.1 µg (lane 1), 0.3 µg (lane 2), and 0.6 µg (lane 3) recombinant human IL23 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-Human IL23A Therapeutic Antibody (Brazikumab) (TAB-008ML) at a dilution of 1:500 overnight at 4°C on a rocking platform. Then probed with SA-HRP secondary antibody. Chemiluminescent was detected.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)

SEC-HPLC

Figure 4 Anti-Human IL23A Therapeutic Antibody (Brazikumab) (TAB-008ML) in DB

Dot Blot analysis of TAB-008ML was performed by coating with human IL4R protein antigen.
TAB-008ML incubation concentration: 2 ng/μL.
The secondary antibody: SA-HRP

ELISA

Figure 5 Anti-Human IL23A Therapeutic Antibody (Brazikumab) (TAB-008ML) in ELISA

ELISA analysis of TAB-008ML was performed by coating with recombinant human IL23a antigen. Then blocked with BSA and incubated with Anti-Human IL23A Therapeutic Antibody (Brazikumab) (TAB-008ML). The SA-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subsets of lymphoid cells in and outside germinal center.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/4856_A_5_8_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/60_H7_1_blue_red_green.jpg

Normal Tissue Figure 4 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/4856_A_9_3.jpg

Normal Tissue Figure 5 Testis

Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/4856_A_5_6.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/4856_A_8_8.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, U-266/70, U-266/84)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110944-IL23A

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
IgG2, λ

Specificity
Human IL23A

Species Reactivity
Human

Applications
ELISA, IHC, FC, IP, IF, Inhib, PK

Conjugate
Unconjugated

CAS
1610353-18-8

Generic Name
Brazikumab

UNII
50EI9G8Q6U

Related Disease
Crohn's disease (CD)

Product Property

Purity
> 95% as determined by SDS-PAGE

Storage
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

Application Notes
The IL23A antibody has been reported in applications of ELISA, IHC, FC, IP, IF, Inhib, PK.

Target

Alternative Names
Interleukin 23 subunit alpha; IL-23A; IL-23 subunit p19; IL23p19

Gene ID
51561

UniProt ID
Q9NPF7

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Breast cancer biomarkers at key points during disease progression

Potent IL23A Inhibition

We utilized this recombinant monoclonal antibody (Brazikumab) in our research to inhibit IL23A signaling in cell-based assays. It demonstrated potent inhibitory effects and is a valuable tool for studying the IL-23 pathway in inflammatory models.

Validated for Multiple Apps

This antibody is quite versatile. We have successfully used it in ELISA, Flow Cytometry, and Immunoprecipitation to detect human IL23A. The quality is consistent, and it helps us dissect the role of this cytokine in memory T cell activation.

Q&As

What is the target of Brazikumab?

A: Brazikumab is a human recombinant monoclonal antibody that targets Human IL23A (Interleukin 23 subunit alpha). It binds to the p19 subunit of IL-23, which is involved in cytokine signaling.
Can this antibody be used to inhibit IL-23 activity?

A: Yes, this antibody is reported for use in Inhibition (Inhib) assays. It is also suitable for ELISA, Immunohistochemistry (IHC), Flow Cytometry (FC), Immunoprecipitation (IP), and Immunofluorescence (IF).

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-008ML, RRID: AB_3111736)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-008ML.pdf

MSDS

MSDS - TAB-008ML.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "Brazikumab"

Afuco™ Anti-IL23A ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-008ML)
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to IL23A. It is a human monoclonal antibody that can be potentially used in the treatment of Crohn's disease (CD).

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