Recombinant Mouse anti-Human DNAJB4 Monoclonal antibody (EML1242)
CAT#: MOB-0551CT
Recombinant Mouse monoclonal antibody to Human DNAJB4, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28383/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28383/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4995/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28385/60951_A_6_6.jpg
Normal Tissue
Figure 5 Adrenal gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28385/61078_B_6_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28385/60951_A_9_3.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BJ, HUVEC TERT2, LHCN-M2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162616-DNAJB4
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Specifications
- Immunogen
- Recombinant full length protein, corresponding to amino acids 1-338 of Human DNAJB4
- Host Species
- Mouse
- Antibody Isotype
- IgG1
- Species Reactivity
- Human
- Clone
- EML1242
- Applications
- Used for immunoassay techniques such as: Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- DNAJB4; DnaJ (Hsp40) homolog, subfamily B, member 4; DjB4; HLJ1; DNAJW; dnaJ homolog subfamily B member 4; HSP40 homolog; heat shock protein 40 homolog; human liver DnaJ-like protein; DnaJ-like heat shock protein 40; heat shock 40 kDa protein 1 homolog;
- Gene ID
- 11080
- UniProt ID
- Q9UDY4
- Protein Refseq
- NP_008965
- Function
- heat shock protein binding; protein binding; unfolded protein binding;
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Accurate Western Blotting
This antibody (clone EML1242) provided excellent results in our Western Blots targeting Human DNAJB4. Since it was raised against the full-length protein, it seems to recognize the native structure very well. We are very happy with the clarity of the results.
Reliable Recombinant Antibody
The consistency of this recombinant mouse antibody is a major plus for our ongoing projects. We store it at -20°C as recommended, and it has maintained its activity over several months. It is a dependable tool for studying this HSP40 family member.
Q&As
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Is the immunogen a peptide or a full-length protein?
A: For this antibody (Clone EML1242), we used a recombinant full-length protein as the immunogen. It corresponds to amino acids 1-338 of the Human DNAJB4 protein, ensuring broad recognition of the target molecule in assays like Western Blot.
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How should I store this antibody upon receipt?
A: The product is shipped at 4°C. Upon delivery, we recommend that you aliquot the antibody and store it at -20°C or -80°C. Avoiding repeated freeze/thaw cycles is critical for maintaining the stability and performance of the antibody.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MOB-0551CT, RRID: AB_3713915)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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|---|---|---|---|
| MOB-3961z | Mouse Anti-DNAJB4 Recombinant Antibody (clone 35D8) | WB, IP, ELISA | Mouse IgG1, κ |
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