Recombinant Mouse anti-Human FCGR3B Monoclonal antibody (NN0383-6M22)
CAT#: MOB-0459CT
Recombinant Mouse monoclonal antibody to Human FCGR3B, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Not detected
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000162747-FCGR3B
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Specifications
- Immunogen
- Human recombinant CD16b extracellular domain.
- Host Species
- Mouse
- Antibody Isotype
- IgG2
- Specificity
- It does not detect CD16a.
- Species Reactivity
- Human
- Clone
- NN0383-6M22
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Lyophilised Reconstitute the antibody with 200 μl sterile PBS to give a final concentration of 0.5mg/ml.
- Buffer
- Preservative: None Constituents: PBS
- Storage
- Store at -70°C
Target
- Alternative Names
- FCGR3B; Fc fragment of IgG, low affinity IIIb, receptor (CD16b); CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb; low affinity immunoglobulin gamma Fc region receptor III-B; fc-gamma RIIIb; fc-gamma RIII-beta; igG Fc receptor III-1; Fc-gamma receptor IIIb (CD 16);
- Gene ID
- 2215
- UniProt ID
- O75015
- Protein Refseq
- NP_000561
- Function
- IgG binding; receptor activity;
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Specificity without CD16a Cross-reactivity
One of the main challenges in our flow cytometry experiments is distinguishing between different Fc fragment receptors. This monoclonal antibody (clone NN0383-6M22) performed exactly as promised, detecting FCGR3B without any cross-reactivity to CD16a. This high level of specificity allowed us to generate very clean and interpretable data for our receptor studies.
Easy Reconstitution and Storage
The lyophilized format of this antibody was very convenient for shipping and storage. Reconstituting it with sterile PBS was straightforward, and the resulting solution remained stable at -70°C as recommended. We used it for Western Blotting of CHO cell lysates expressing the human recombinant CD16b extracellular domain, obtaining strong signal detection.
Q&As
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Does this antibody cross-react with CD16a?
A: No, this Recombinant Mouse anti-Human FCGR3B Monoclonal antibody (clone NN0383-6M22) does not detect CD16a. It is specific for Human FCGR3B (CD16b) and has been tested to ensure it distinguishes between these related receptors. The immunogen used was the Human recombinant CD16b extracellular domain expressed in CHO cells.
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How should I reconstitute and store this product?
A: This product is supplied as a lyophilized powder. We recommend reconstituting the antibody with 200 μl of sterile PBS to achieve a final concentration of 0.5 mg/ml. For storage, the reconstituted antibody should be kept at -70°C to maintain stability. It contains PBS but no preservatives, so sterile handling is essential.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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