Recombinant Mouse anti-Human POLQ Monoclonal antibody (1C11)
CAT#: MOB-0547CT
Recombinant Mouse monoclonal antibody to Human POLQ, expressed in Chinese Hamster Ovary cells(CHO).
Specifications
- Immunogen
- A recombinant fragment of human DNA Polymerase theta, encompassing amino acid residues 1609 to 1717, was used as the immunogen.
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 1C11
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein A purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Preservative: None Constituents: 1X PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- POLQ; polymerase (DNA directed), theta; POLH; PRO0327; DNA polymerase theta; DNA polymerase eta;
- Gene ID
- 10721
- UniProt ID
- O75417
- Protein Refseq
- NP_955452
- Function
- ATP binding; ATP-dependent helicase activity; DNA-directed DNA polymerase activity; damaged DNA binding; helicase activity; nucleotide binding; nucleotidyltransferase activity; single-stranded DNA-dependent ATPase activity; transferase activity;
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Accurate Detection Of POLQ
This recombinant mouse monoclonal antibody was essential for our DNA polymerase theta research. It showed excellent specificity in ELISA assays, reacting strongly with the recombinant fragment immunogen we used as a positive control in our setup.
High Quality Recombinant Antibody
The consistency of this recombinant antibody is superior to traditional hybridoma products we have used. We obtained clean Western Blot results targeting Human POLQ, which helped us validate the expression levels in our CHO cell expression system.
Q&As
-
What is the specific immunogen for this antibody?
A: This antibody was raised against a recombinant fragment corresponding to amino acids 1609-1717 of Human DNA Polymerase theta (POLQ). It effectively recognizes this specific region of the protein in your assays.
-
What is the host species and expression system?
A: This is a Recombinant Mouse monoclonal antibody (clone 1C11) expressed in Chinese Hamster Ovary (CHO) cells. The recombinant expression ensures batch-to-batch consistency and reliability for applications like ELISA and Western Blot.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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