Recombinant Mouse anti-Human ZNF397 Monoclonal antibody (1F8)
CAT#: MOB-2169CT
Recombinant Mouse monoclonal antibody to Human ZNF397, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli, plasma membrane, cytosol & microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26087/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26087/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, plasma membrane, cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26087/279_E8_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26087/55943_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26087/55943_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26087/55943_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26087/55943_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26087/55943_A_4_6.jpg
Normal Tissue
Figure 9 Breast
Adipocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Myoepithelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26087/55943_B_2_4.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26087/55943_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186812-ZNF397
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Specifications
- Immunogen
- The immunogen is a recombinant fragment of human ZNF397, corresponding to amino acids 131-230, with a proprietary tag. The specific sequence of the fragment is: EFDDPGQQVP ASPQGPAVPW KDLTCLRASQ ESTDIHLQPL KTQLKSWKPC LSPKSDCENS ETATKEGISE EKSQGLPQEP SFRGIKLSRP PKASSAIRWE.
- Host Species
- Mouse
- Antibody Isotype
- IgG2a
- Species Reactivity
- Human
- Clone
- 1F8
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein A purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Preservative: None Constituents: 1X PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- ZNF397; zinc finger protein 397; ZNF47; ZSCAN15; zinc finger protein 47; zinc finger and SCAN domain-containing protein 15;
- Gene ID
- 84307
- UniProt ID
- Q8NF99
- Protein Refseq
- NP_001128650
- Function
- DNA binding; metal ion binding; sequence-specific DNA binding transcription factor activity; zinc ion binding;
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "ZNF397"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-787 | Human Anti-ZNF397 Recombinant Antibody (clone T80) | IP, IF, ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2542 | Anti-ZNF397 Immunohistochemistry Kit | IHC |
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