What are the primary research applications of the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody?

The Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody is primarily used to study the pathogenesis of Listeria monocytogenes. Researchers employ this antibody to investigate bacterial infection mechanisms, host-pathogen interactions, and the immune response to Listeria infections. It is a valuable tool in microbiology and immunology research.

How should the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody be stored to maintain its stability?

To ensure stability, the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody should be stored at -20°C or lower. Aliquoting the antibody is recommended to prevent repeated freeze-thaw cycles. Thaw aliquots on ice and gently mix before use. Proper storage conditions are essential for preserving antibody activity.

What are the recommended concentrations and conditions for using this antibody in enzyme-linked immunosorbent assays (ELISA)?

For ELISA, it is recommended to use the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody at a concentration of 1-2 µg/mL. Coating the ELISA plates with antigen overnight at 4°C, followed by blocking with a suitable buffer, and then incubating with the antibody for 1-2 hours at room temperature ensures optimal results.

How does the specificity of the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody affect its use in detecting Listeriolysin O in complex samples?

The high specificity of this antibody to Listeriolysin O ensures accurate detection of LLO in complex samples such as bacterial lysates or infected host cells. This specificity reduces cross-reactivity with other proteins, providing clear and reliable results in various assay formats.

Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody (M275)

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See other products for "L. monocytogenes EGD-e LLO"

CAT	Product Name	Application	Type
EPAF-1066LC	Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody (M344)	BL, ELISA	IgG2b
EPAF-1068LC	Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody (S179)	BL, ELISA	IgG1

CAT

Product Name

Application

Type

EPAF-1066LC

Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody (M344)

BL, ELISA

IgG2b

EPAF-1068LC

Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody (S179)

BL, ELISA

IgG1

Customer Reviews and Q&As

CAT	Product Name	Application	Type
MHC-CN0313	APC-H-2Kb/Human Listeriolysin O (VAYGRQVYL) MHC Tetramer	FCM
MHC-CN0314	PE-H-2Kb/Human Listeriolysin O (VAYGRQVYL) MHC Tetramer	FCM

Customer Review Q&As

Exceptional Specificity in Pathogen Research

The Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody excels in pathogen research. Its outstanding specificity ensures accurate detection of Listeria, significantly enhancing the quality of microbial studies.

Consistent Performance in Diagnostic Development

This antibody ensures exceptional consistency, crucial for diagnostic development. It offers reliable results in detecting Listeria monocytogenes, significantly improving diagnostic accuracy and efficiency.

Q 1: What are the primary research applications of the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody?
A: The Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody is primarily used to study the pathogenesis of Listeria monocytogenes. Researchers employ this antibody to investigate bacterial infection mechanisms, host-pathogen interactions, and the immune response to Listeria infections. It is a valuable tool in microbiology and immunology research.
Q 2: How should the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody be stored to maintain its stability?
A: To ensure stability, the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody should be stored at -20°C or lower. Aliquoting the antibody is recommended to prevent repeated freeze-thaw cycles. Thaw aliquots on ice and gently mix before use. Proper storage conditions are essential for preserving antibody activity.
Q 3: What are the recommended concentrations and conditions for using this antibody in enzyme-linked immunosorbent assays (ELISA)?
A: For ELISA, it is recommended to use the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody at a concentration of 1-2 µg/mL. Coating the ELISA plates with antigen overnight at 4°C, followed by blocking with a suitable buffer, and then incubating with the antibody for 1-2 hours at room temperature ensures optimal results.
Q 4: How does the specificity of the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody affect its use in detecting Listeriolysin O in complex samples?
A: The high specificity of this antibody to Listeriolysin O ensures accurate detection of LLO in complex samples such as bacterial lysates or infected host cells. This specificity reduces cross-reactivity with other proteins, providing clear and reliable results in various assay formats.
Q 5: What steps should be taken if the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody shows non-specific binding in experiments?
A: To address non-specific binding, optimize antibody concentration and blocking conditions, use appropriate controls, and ensure thorough washing steps. Validating the antibody specificity in the experimental context and optimizing sample preparation can also help reduce non-specific signals.