Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody (M275)

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Q&As

1. What are the primary research applications of the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody?

   A: The Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody is primarily used to study the pathogenesis of Listeria monocytogenes. Researchers employ this antibody to investigate bacterial infection mechanisms, host-pathogen interactions, and the immune response to Listeria infections. It is a valuable tool in microbiology and immunology research.

2. How should the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody be stored to maintain its stability?

   A: To ensure stability, the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody should be stored at -20°C or lower. Aliquoting the antibody is recommended to prevent repeated freeze-thaw cycles. Thaw aliquots on ice and gently mix before use. Proper storage conditions are essential for preserving antibody activity.

3. What are the recommended concentrations and conditions for using this antibody in enzyme-linked immunosorbent assays (ELISA)?

   A: For ELISA, it is recommended to use the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody at a concentration of 1-2 µg/mL. Coating the ELISA plates with antigen overnight at 4°C, followed by blocking with a suitable buffer, and then incubating with the antibody for 1-2 hours at room temperature ensures optimal results.

4. How does the specificity of the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody affect its use in detecting Listeriolysin O in complex samples?

   A: The high specificity of this antibody to Listeriolysin O ensures accurate detection of LLO in complex samples such as bacterial lysates or infected host cells. This specificity reduces cross-reactivity with other proteins, providing clear and reliable results in various assay formats.

5. What steps should be taken if the Recombinant Mouse Anti-L. monocytogenes EGD-e LLO Antibody shows non-specific binding in experiments?

   A: To address non-specific binding, optimize antibody concentration and blocking conditions, use appropriate controls, and ensure thorough washing steps. Validating the antibody specificity in the experimental context and optimizing sample preparation can also help reduce non-specific signals.

View the frequently asked questions answered by Creative Biolabs Support.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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