Mouse Anti-NP Recombinant Antibody (clone N1G9) (CAT#: PABW-094)

Cao, Fengqiang, et al. "Inside-out assembly of viral antigens for the enhanced vaccination." Signal transduction and targeted therapy 8.1 (2023): 189. https://doi.org/10.1038/s41392-023-01414-7

This research introduces an innovative "inside-out assembly" approach for enhanced vaccination against enveloped RNA viruses. The study developed a multi-layered aluminum hydroxide-stabilized emulsion (MASE) that reverses the natural viral packaging strategy by placing nucleocapsid protein (NP) on the outside of droplets while trapping the receptor-binding domain (RBD) inside. This reversed delivery sequence triggers potent type I interferon-mediated immune responses in advance, creating an immune-potentiated environment that subsequently boosts dendritic cell activation and lymph node engagement. When tested with both H1N1 influenza and SARS-CoV-2 antigens, this inside-out strategy significantly increased antibody secretion, memory T cell engagement, and Th1-biased immune responses, effectively reducing viral loads after lethal challenge.
Creative Biolabs contributed anti-NP antibodies that were essential for verifying the structural design of the vaccine formulation. These antibodies were used to confirm the proper spatial arrangement of antigens in the multi-layered structure through confocal imaging. This enabled researchers to visually validate that NP was indeed displayed on the surface while other antigens remained entrapped inside the nanocage, confirming the successful implementation of the inside-out strategy that formed the foundation of this study's enhanced immune response.