Recombinant Human Anti-Nicotine Antibody scFv Fragment (Nic12) (CAT#: PABX-147-S (P))

Anti-Nicotine antibody is a Human of scFv class that binds to an Nicotine.


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Figure 1 Characterization of nicotine-specific antibodies.

Figure 1 Characterization of nicotine-specific antibodies.

(A) Inhibition of nicotine entry into brain. Mice were injected i.p. with 0.5 mg of the indicated human IgG2. One day later, mice were injected i.v. with 750 ng of tritium-labeled
nicotine. Mice were killed after 5 min and nicotine concentrations in brains were measured. Individual mice are shown; bars indicate average values. IgG, control human IgG. *, P < 0.001 vs. IgG; **, P < 0.003 vs. IgG. (B) Dose-response of inhibition of nicotine entry into brain by IgG2-Nic12. Mice were injected i.p. with 0.1, 0.3, 1, or 3mg of mAb-Nic12. One day later, mice were injected i.v. with 750 ng of tritium-labeled nicotine. Mice were killed after 5 min and nicotine concentrations in brains were measured. Antibody concentrations in sera were determined and plotted against the percentage nicotine-reduction in the brain. (C) Time course of inhibition of nicotine entry into brain by IgG2-Nic12. Mice were injected with 0.5mgantibody, challenged with750ngof tritium-labeled nicotine and nicotine in the brain was measured at the indicated times. Averages of 4 to 5 mice are given with standard deviations. IgG, control human IgG. *, P < 0.001 vs. IgG. (D) Specificity of mAb-Nic12. The binding of tritium-labeled-nicotine (56 nM) to mAb-Nic12 (50 nM) was measured by equilibrium dialysis in the presence of increasing concentrations of unlabeled (-)-nicotine, (-)-cotinine, or acetylcholine. Averages of two independent experiments are given with standard deviations.

Beerli, R. R., Bauer, M., Buser, R. B., Gwerder, M., Muntwiler, S., Maurer, P., ... & Bachmann, M. F. (2008). Isolation of human monoclonal antibodies by mammalian cell display. Proceedings of the National Academy of Sciences, 105(38), 14336-14341.


Specifications

  • Immunogen
  • Nicotine-carrier protein conjugate vaccine
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • scFv
  • Specificity
  • Tested positive against native Human VEGF
  • Clone
  • Nic12
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:10000-1:20000
    Neut (assay dependent)
    FuncS (assay dependent)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Human Antibody

CAT Product Name Application Type
PABX-147 Recombinant Human Anti-Nicotine Antibody (Nic12) WB, ELISA, Neut, FuncS IgG
PABX-147-F (E) Recombinant Human Anti-Nicotine Antibody Fab Fragment (Nic12) WB, ELISA, Neut, FuncS Fab

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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