Rat Anti-PA Tag Recombinant Antibody (clone NZ-1) (CAT#: PABL-673)
Recombinant Rat Antibody (NZ-1) is capable of binding to PA tag. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody.
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Figure 1 Flow cytometric analysis of PA-tagged membrane protein.
CHO-K1 cells (gray area) or CHO-K1 cells transiently transfected with PA12-rNrp2-mCherry (solid line) were incubated with 10 ug/ml NZ-1 and stained with FITC-labeled secondary antibody specific for rat IgG.
Fujii, Y., Kaneko, M., Neyazaki, M., Nogi, T., Kato, Y., & Takagi, J. (2014). PA tag: a versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin. Protein expression and purification, 95, 240-247.
Figure 2 Western blot analysis of PA tagged IDH1 with NZ-1.
Membranes were incubated with varying concentrations of NZ-1 for 30 min. All steps after the NZ-1 incubation, including secondary antibody reaction and chemiluminescence visualization, were conducted under the exactly same condition for all membranes.
Fujii, Y., Kaneko, M., Neyazaki, M., Nogi, T., Kato, Y., & Takagi, J. (2014). PA tag: a versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin. Protein expression and purification, 95, 240-247.
Specifications
- Immunogen
- Human PA tag
- Host Species
- Rat
- Derivation
- Rat
- Type
- Rat IgG
- Specificity
- Human PA Tag
- Species Reactivity
- Human
- Clone
- NZ-1
- Applications
- WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- PA tag
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-667 | Rat Anti-PA Tag Recombinant Antibody (clone NZ-1); Fab Fragment | WB, FuncS | Rat Fab |
Customer Reviews and Q&As
Customer Review
S***n
Reliable and Specific for PA Tag Detection in Western Blot
Reliable and Specific for PA Tag Detection in Western Blot
We've been using the Rat Anti-PA Tag Recombinant Antibody (clone NZ-1) (CAT#: PABL-673) from Creative Biolabs in our Western blot experiments, and it has delivered excellent results. The antibody shows great specificity for the PA tag with minimal background noise. It provides clear, strong bands even at low expression levels, making it perfect for our recombinant protein studies. We've also noticed good reproducibility between different lots, which has been essential for consistent data. This antibody has become a staple in our lab, and I highly recommend it for anyone working with PA-tagged proteins.
19.Jul,24
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