Hi-Affi™ Recombinant Rabbit Anti-PLA2G2A Monoclonal Antibody (DS2732AB)
CAT#: MOR-2732
Recombinant Rabbit anti-PLA2G2A monoclonal antibody for IHC-P, IP, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human duodenum
Immunohistochemical staining of human testis shows high expression in duodenum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15236/41162_A_8_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15236/41162_B_7_5.jpg
Normal Tissue
Figure 3 Small intestine
Paneth cells
Staining:High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15236/41162_A_5_2.jpg
Normal Tissue
Figure 4 Colon
Mucosal lymphoid cells
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15236/41162_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15236/41162_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Preleptotene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15236/41162_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (Hep G2, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188257-PLA2G2A/subcellular
❮
❯
Specifications
- Immunogen
- Human recombinant sPLA2 (Type IIa).
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Human
- Clone
- DS2732AB
- Applications
- Used for immunoassay techniques such as: Immunohistochemistry (Paraffin); Immunoprecipitation; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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High-Quality Monoclonal
The Recombinant Rabbit Anti-PLA2G2A monoclonal antibody exhibits excellent quality with>95% purity. Its performance in IHC-P, IP, and Western blot experiments has been consistent. I appreciate the detailed product literature which clarifies the purification process using Absolute Mag Protein A Agarose and gives clear storage guidelines for my research.
Reliable and Detailed
I am impressed with this Hi-Affi™ rabbit antibody for PLA2G2A detection. The comprehensive details regarding its purification, high specificity, and recommended storage conditions have significantly benefited my research projects. This reagent supports reliable immunoassays, and the provided data helps in achieving reproducible and accurate experimental outcomes.
Q&As
-
What are the advantages of this Hi-Affi™ recombinant antibody for PLA2G2A detection?
A: We developed this recombinant rabbit antibody to specifically target PLA2G2A with high purity and specificity. Its production using phage display and purification via Absolute Mag Protein A Agarose result in excellent performance in IHC-P, IP, and Western blot assays, offering a consistent and reliable research reagent.
-
How does the purification process influence its performance in research assays?
A: Our antibody is purified under strict quality standards resulting in>95% purity. This meticulous purification process ensures that the recombinant rabbit antibody exhibits consistent binding to PLA2G2A in various assays, including IHC-P and IP, thereby enhancing the reproducibility and reliability of your research findings.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1823z | Mouse Anti-PLA2G2A Recombinant Antibody (clone 30C11) | WB, IP | Mouse IgG2b |
| CAT | Product Name | Application | Type |
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| TAB-1084CL | Mouse Anti-PLA2G2A Recombinant Antibody (TAB-1084CL) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1085CL | Mouse Anti-PLA2G2A Recombinant Antibody (TAB-1085CL) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1084CL-S(P) | Mouse Anti-PLA2G2A Recombinant Antibody; scFv Fragment (TAB-1084CL-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1085CL-S(P) | Mouse Anti-PLA2G2A Recombinant Antibody; scFv Fragment (TAB-1085CL-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1084CL-F(E) | Mouse Anti-PLA2G2A Recombinant Antibody; Fab Fragment (TAB-1084CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0422-YC | Human Anti-PLA2G2A Recombinant Antibody (HPAB-0422-YC) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0423-YC | Human Anti-PLA2G2A Recombinant Antibody (HPAB-0423-YC) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0424-YC | Human Anti-PLA2G2A Recombinant Antibody (HPAB-0424-YC) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0422-YC-S(P) | Human Anti-PLA2G2A Recombinant Antibody; scFv Fragment (HPAB-0422-YC-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0423-YC-S(P) | Human Anti-PLA2G2A Recombinant Antibody; scFv Fragment (HPAB-0423-YC-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0424-YC-S(P) | Human Anti-PLA2G2A Recombinant Antibody; scFv Fragment (HPAB-0424-YC-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0422-YC-F(E) | Human Anti-PLA2G2A Recombinant Antibody; Fab Fragment (HPAB-0422-YC-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0423-YC-F(E) | Human Anti-PLA2G2A Recombinant Antibody; Fab Fragment (HPAB-0423-YC-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0424-YC-F(E) | Human Anti-PLA2G2A Recombinant Antibody; Fab Fragment (HPAB-0424-YC-F(E)) | ELISA | Human Fab |
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| VS-0525-XY5454 | Anti-PLA2G2A Immunohistochemistry Kit | IHC |
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| VS-0625-YC258 | Recombinant Anti-PLA2G2A Eliminating Antibody, pH-Sensitive (VS-0625-YC258) | Antigen-Sweeping In Vivo. | Human IgG |
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