Rabbit Anti-POLR3F Recombinant Antibody (MOB-3513z)
CAT#: MOB-3513z
The purified Rabbit Anti-POLR3F Recombinant Antibody (MOB-3513z) product displayed high specificity against the POLR3F protein of Human.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72298/1417_H6_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49441/114552_A_5_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49441/114625_B_9_5.jpg
Normal Tissue
Figure 4 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49441/114625_B_3_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Not detected
Intensity: Negative
Quantity: None
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49441/114552_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49441/114552_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49441/114552_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49441/114552_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49441/114552_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132664-POLR3F/subcellular
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❯
Specifications
- Immunogen
- A synthetic peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human POLR3F
- Species Reactivity
- Human
- Applications
- WB
- Conjugate
- Unconjugated
- MW
- 36 kDa
- Related Disease
- Immunodeficiency 101 and Corneal Dystrophy, Posterior Polymorphous, 1
Product Property
- Clonality
- Monoclonal antibody
- Purification
- Protein A purified
- Format
- Liquid
- Buffer
- PBS, 40% Glycerol and 0.05% BSA
- Preservative
- 0.01% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Cold Packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot. Specific conditions should be optimized by the end user.
Western Blot (1:1000-1:10000)
Target
- Alternative Names
- POLR3F; RNA Polymerase III Subunit C6; RPC6; RNA Polymerase III C39 Subunit; Polymerase (RNA) III (DNA Directed) Polypeptide F (39 KDa); RNA Polymerase III 39 KDa Subunit; DNA-Directed RNA Polymerase III Subunit F; RPC39; Polymerase (RNA) III (DNA Directe
- Gene ID
- 10621
- UniProt ID
- Q9H1D9
- Long Name
- RNA Polymerase III Subunit F
- Protein Refseq
- NP_006457.2
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Strong Signals In Western Blot
We tested this rabbit anti-POLR3F antibody in Western Blot at a dilution of 1:1000 and obtained very strong signals. It specifically recognized the human POLR3F protein, a subunit of RNA Polymerase III. The antibody was purified by Protein A and showed no non-specific binding.
Specific Peptide Immunogen
The specificity of this antibody is likely due to the synthetic peptide immunogen used. It accurately detected the 39 kDa subunit of RNA Polymerase III in our samples. We followed the storage instructions, keeping it at 4°C for short-term use, and it remained stable and effective.
Q&As
-
What is the target protein complex for this antibody?
A: The target POLR3F is one of the subunits forming eukaryotic RNA polymerase III (RNA Pol III). This enzyme transcribes 5S ribosomal RNA and tRNA genes. POLR3F is unique to this polymerase and binds to TFIIIB90 and TBP subunits.
-
What is the composition of the buffer provided with the antibody?
A: The antibody is supplied in a buffer containing PBS, 40% Glycerol, and 0.05% BSA, with 0.01% sodium azide as a preservative. This formulation is designed to maintain stability when stored at -20°C for long-term use.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "POLR3F"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3513z-F(E) | Recombinant Anti-Human POLR3F Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3513z-S(P) | Recombinant Anti-Human POLR3F Antibody scFv Fragment | Neut, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2786 | Hi-Affi™ Recombinant Rabbit Anti-POLR3F Monoclonal Antibody (DS2786AB) | WB | IgG |
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