Hi-Affi™ Recombinant Rabbit Anti-PRKAA2 Monoclonal Antibody (DS2845AB) (CAT#: MOR-2845)
Recombinant Rabbit anti-PRKAA2 monoclonal antibody for ELISA, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/1581_D4_3_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/1581_D4_3_selected.jpg
Figure 2 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_B_6_6_selected.jpg
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_B_6_6_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_B_8_5.jpg
(Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_B_8_5.jpg
Figure 4 Colon
(Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_A_8_3.jpg
(Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_A_8_3.jpg
Figure 5 Kidney
(Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_A_7_5.jpg
(Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_A_7_5.jpg
Figure 6 Testis
(Leydig cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_A_6_6.jpg
(Leydig cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_A_6_6.jpg
Figure 7 Skeletal muscle
(Myocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_B_2_6.jpg
(Myocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_B_2_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (RPTEC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162409-PRKAA2/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162409-PRKAA2/subcellular
Specifications
- Immunogen
- Synthetic phospho-peptide corresponding to N-terminal residues (phospho site at 175 Serine) of human AMPK alpha 2 protein.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Human
- Clone
- DS2845AB
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0519U | Rabbit Anti-Phospho-PRKAA2 (Thr172) Recombinant Antibody (clone 4F4) | ELISA, WB, IF | Rabbit IgG |
| ZG-0520U | Rabbit Anti-Phospho-PRKAA2 (S491) Recombinant Antibody (clone 3H10) | ELISA, WB | Rabbit IgG |
| VS3-QX915 | Mouse Anti-PRKAA2 Recombinant Antibody (clone 4B11-G3-G10) | WB, ICC | Mouse IgG2a, κ |
| VS3-WK170 | Mouse Anti-PRKAA2 Recombinant Antibody (clone H5-E11) | WB, ELISA | Mouse IgG1 |
| VS3-CJ1038 | Rabbit Anti-PRKAA2 (phospho S345) Recombinant Antibody (VS3-CJ1038) | WB, ICC, IF, IHC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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