Recombinant Mouse Anti-RAV VP7 Antibody (4F8) (CAT#: PABZ-109)
Recombinant Mouse Antibody (4F8) is capable of binding to RAV VP7, expressed in Chinese Hamster Ovary cells (CHO).
Specific Inquiry
Figure 1 Analysis by agarose gel electrophoresis of VP7-DLP association at high and low free calcium concentrations and in the presence or absence of VP7-binding MAbs or Fabs.
The fluorescence of 488-labeled VP7 is green; that of ethidium bromide (EtBr)-stained DLPs is red. (A) Calcium dependence of uncoating in the absence of MAbs or Fabs. Free calcium concentrations vary from 147 to 2 nM. Controls are DLPs alone and 488-labeled VP7 alone (two lanes on the right). Note the separation of the EtBr signal and VP7 signal at low calcium concentrations as DLP migration slows and VP7 migration accelerates. (B) Effects of 4F8 (region 7-1) MAb and Fab on uncoating. MAb and Fab concentrations vary from 1.6 to 0.2 μM. Samples are in 147 nM free calcium (+) or 21 nM free calcium (-). Note the retention of both DLP and VP7 signals in the wells in the presence of 4F8 MAbs, regardless of calcium concentration, and the associated DLP and VP7 signals in the presence of sufficient 4F8 Fab and high or low free calcium concentrations. "VP7 + DLP" lanes contain recoated particles supplemented with buffer alone. (C) Effect of RV3:4 (region 7-2) MAb and Fab on uncoating. MAb and Fab concentrations vary from 4.8 to 0.6 μM. At low free calcium concentrations, the highest RV3:4 MAb concentrations reduce the DLP signal from dissociated VP7. Even at the highest RV3:4 Fab concentrations, separation of the DLP and VP7 signals is essentially complete in low free calcium.
Aoki, S. T., Trask, S. D., Coulson, B. S., Greenberg, H. B., Dormitzer, P. R., & Harrison, S. C. (2011). Crosslinking rotavirus outer capsid protein VP7 by antibodies or disulfides inhibits viral entry. Journal of virology, JVI-00234.
Figure 2 Antigenicity of the wild-type and disulfide cross-linked VP7 trimer, measured by ELISA.
x axis corresponds to the antibody concentration tested. (A) Wild-type VP7 (VP7 wt) in PBS. (B) Wild-type VP7 in PBS plus 0.9 mM CaCl₂. (C) Disulfide-cross-linked VP7 (VP7 S-S) in PBS. (D) Disulfide cross-linked VP7 in PBS plus 0.9 mM CaCl₂.
Aoki, S. T., Trask, S. D., Coulson, B. S., Greenberg, H. B., Dormitzer, P. R., & Harrison, S. C. (2011). Crosslinking rotavirus outer capsid protein VP7 by antibodies or disulfides inhibits viral entry. Journal of virology, JVI-00234.
Specifications
- Immunogen
- RVA VP7
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native RAV VP7
- Species Reactivity
- RVA
- Clone
- 4F8
- Applications
- ELISA Assay-Dependent
Neut Assay-Dependent
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody 4F8 has been reported in applications of ELISA, Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
ELISA: It was reported that 4F8 was bound to 96-well plates at 1 μg/well (10 μg/ml) in PBS overnight for ELISA.
Target
- Alternative Names
- VP7; RAV VP7
- Gene ID
- 7011359
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-109 | Recombinant Mouse Anti-RAV VP7 Antibody Fab Fragment (4F8) | Neut | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-109 | Recombinant Mouse Anti-RAV VP7 Antibody scFv Fragment (4F8) | Neut | scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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