Recombinant Human Anti-S. aureus Antibody (4497) (CAT#: PABL-328)
Recombinant Human Antibody (4497) is capable of binding to S. aureus, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-S. aureus mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-S. aureus mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specific Inquiry
Figure 1 Antibody binding to S. aureus is dependent on WTA glycosyltransferases.
Protein A-deficient USA300 S. aureus wild type, ΔtarM, and ΔtarS strains were incubated with anti-WTA (red) or isotype control (grey; human IgG1 anti-cytomegalovirus gD antigen) antibodies, followed by detection with a fluorescently-labeled (DyLight 649) secondary antibody. Stained cells were counted by flow cytometry and data visualized in the displayed histograms. The 4497 antibodies bound to wild type and ΔtarM strains.
Fong, R., Kajihara, K., Chen, M., Hotzel, I., Mariathasan, S., Hazenbos, W. L., & Lupardus, P. J. (2018, October). Structural investigation of human S. aureus-targeting antibodies that bind wall teichoic acid. In mAbs (Vol. 10, No. 7, pp. 979-991). Taylor & Francis.
Figure 2 Analysis of anti-WTA antibody binding to S. aureus.
Protein A-deficient USA300 S. aureus were incubated with ¹²⁵I-labelled anti-WTA antibodies and subjected to immunoradiometric assay and saturation binding analysis to determine antibody affinity. Titration curves are shown for 4497, along with calculated KD values and estimated number of sites/bacterium.
Fong, R., Kajihara, K., Chen, M., Hotzel, I., Mariathasan, S., Hazenbos, W. L., & Lupardus, P. J. (2018, October). Structural investigation of human S. aureus-targeting antibodies that bind wall teichoic acid. In mAbs (Vol. 10, No. 7, pp. 979-991). Taylor & Francis.
Specifications
- Immunogen
- S. aureus Staphylococcus aureus
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native S. aureus
- Species Reactivity
- S. aureus
- Clone
- 4497
- Applications
- Can be useful in applications such as: Western blot; Enzyme-linked Immunosorbent Assay; Functional Study
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- S. aureus; Staphylococcus aureus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-870 | Recombinant Anti-S. aureus Antibody | WB, IP, FuncS | IgG |
| MHH-870 | Recombinant Human Anti-S. aureus Antibody | IP, IF, Biosensors, FuncS | IgG |
| HPAB-M0204-YC | Recombinant Bovine Anti-S. aureus Antibody (M/YC-23) | ELISA | Bovine IgG |
| HPAB-A1223-YJ | Human Anti-S. aureus Recombinant Antibody (clone scfv88) | ELISA, Inhib, FuncS | Chimeric (bovine/human) IgG1 |
| HPAB-A1224-YJ | Human Anti-S. aureus Recombinant Antibody (clone scFv73) | ELISA, Inhib | Chimeric (bovine/human) IgG1 |
Fab Fragment Antibody
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-870-S(P) | Recombinant Anti-S. aureus Antibody scFv Fragment | WB, IF, FuncS | scFv |
| MHH-870-S(P) | Recombinant Human Anti-S. aureus Antibody scFv Fragment | WB, ELISA, IHC, FuncS | scFv |
| PSBL-328 | Recombinant Human Anti-S. aureus Antibody scFv Fragment (4497) | WB, ELISA, FuncS | scFv |
| HPAB-M0204-YC-S(P) | Recombinant Bovine Anti-S. aureus Antibody scFv Fragment (M/YC-23) | ELISA | Bovine scFv |
| HPAB-0585-YJ-S(P) | Bovine Anti-S. aureus Recombinant Antibody; scFv Fragment (HPAB-0585-YJ-S(P)) | ELISA | Bovine scFv |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-033 | Recombinant Anti-S. aureus VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-058MZ | Human Anti-S. aureus Recombinant Antibody (MRO-058MZ) | ELISA | Human IgG1 |
| MRO-059MZ | Human Anti-S. aureus Recombinant Antibody (MRO-059MZ) | ELISA | Human IgG1 |
| MRO-060MZ | Human Anti-S. aureus Recombinant Antibody (MRO-060MZ) | ELISA | Human IgG1 |
| MRO-061MZ | Human Anti-S. aureus Recombinant Antibody (MRO-061MZ) | ELISA | Human IgG1 |
| MRO-058MZ-F(E) | Human Anti-S. aureus Recombinant Antibody; Fab Fragment (MRO-058MZ-F(E)) | ELISA | Human Fab |
Mouse Antibody
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-167-S (P) | Recombinant Human Anti-S. aureus Antibody scFv Fragment (LTM14) | Neut, ELISA, FuncS | scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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