Recombinant Mouse Anti-SARS-CoV S Antibody ( F26G19) (CAT#: PABW-114)

Recombinant Mouse Antibody ( F26G19) is capable of binding to SARS-CoV S, expressed in Chinese Hamster Ovary cells (CHO).


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Figure 1 ELISA reactivity of mAbs with whole, inactivated SARS-CoV.

Figure 1 ELISA reactivity of mAbs with whole, inactivated SARS-CoV.

Hybridoma supernatants were tested at a 1:4 dilution in PBS + 0.2% BSA on pre-blocked plates, coated with 18 ng per well of inactivated, highly purified SARS-CoV. Positive clones were identified as having positive binding (color) in wells that were at least four-fold higher than the background level reactivity on BSA. Antigen legend: black bars—native, highly purified SARS-CoV; white bars—heat denatured, highly purified SARS-CoV; grey bars—BSA control.

Berry, J. D., Jones, S., Drebot, M. A., Andonov, A., Sabara, M., Yuan, X. Y., ... & Nicolas, B. (2004). Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus. Journal of virological methods, 120(1), 87-96.

Figure 2 Western immunoblot reactivity of mAbs with highly purified SARS-CoV.

Figure 2 Western immunoblot reactivity of mAbs with highly purified SARS-CoV.

The positive and preimmune control sera were from the corresponding immune mouse and tested at 1:2000 dilution in TBS + 0.2% BSA. Lanes marked 1 were loaded with highly purified SARS-CoV, lanes marked 2 with infected Vero cell lysate. The molecular weights of the pre-stained kaleidoscope markers (BioRAD) are indicated on the left of the figure.

Berry, J. D., Jones, S., Drebot, M. A., Andonov, A., Sabara, M., Yuan, X. Y., ... & Nicolas, B. (2004). Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus. Journal of virological methods, 120(1), 87-96.


Specifications

  • Immunogen
  • SARS-CoV E2 glycoprotein precursor
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native SARS-CoV S
  • Species Reactivity
  • SARS-CoV
  • Clone
  • F26G19
  • Applications
  • WB, ELISA

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The SARS-CoV S antibody has been reported in applications of ELISA, WB.

Target

  • Alternative Names
  • E2; S; E2 glycoprotein precursor; SARS-CoV S

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Epitope-Specific Antibody

CAT Product Name Application Type
EPAF-1139CQ Recombinant Human Anti-SARS-CoV S Antibody (m396) ELISA IgG1

Recombinant Antibody

Single-domain Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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