Rabbit Anti-T7 RNA Polymerase Antibody (CAT#: MOB-0296MC)
The product is a polyconal antibody that was generated in rabbit immunized with full length T7 RNA polymerase. The antibody was validated in ELISA, western blot and dot blot assays that specifically detect recombinant T7 RNA polymerase. The anti-T7 RNA polymerase antibody is reliably available and ready to ship.
Specific Inquiry
SDS-PAGE
Figure 1 Rabbit Anti-T7 RNA Polymerase Antibody (MOB-0296MC) in SDS-PAGE
Lane 1: Reduced antibody
ELISA
Figure 2 Rabbit Anti-T7 RNA Polymerase Antibody (MOB-0296MC) in ELISA
ELISA analysis of MOB-0296MC was performed by coating with T7 RNA polymerase protein.
The secondary antibody: HRP Goat Anti-Rabbit IgG
WB
Figure 3 Rabbit Anti-T7 RNA Polymerase Antibody, HRP (MOB-0296MC-HRP) in WB
Western blot analysis of MOB-0296MC-HRP was performed by loading T7 RNA polymerase protein.
MOB-0296MC-HRP incubation concentration: 2 ng/μL.
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
ELISA
Figure 4 Rabbit Anti-T7 RNA Polymerase Antibody, HRP (MOB-0296MC-HRP) in ELISA
ELISA analysis of MOB-0296MC-HRP was performed by coating with T7 RNA polymerase protein.
DB
Figure 5 Rabbit Anti-T7 RNA Polymerase Antibody, HRP (MOB-0296MC-HRP) in Dot Blot
Dot Blot analysis of MOB-0296MC-HRP was performed by coating with T7 RNA polymerase protein.
MOB-0296MC-HRP incubation concentration: 2 ng/μL.
Specifications
- Immunogen
- Recombinant T7 RNA Polymerase ( full-length)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Applications
- Used for immunoassay techniques such as: Enzyme-linked immunosorbent assay; Western Blot
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration
- Buffer
- PBS 7.4 with 0.2M glycine
- Preservative
- No Preservative
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- T7 RNA Polymerase
Related Resources
Dukhovny, Anna, Amir Shlomai, and Ella H. Sklan. "The antiviral protein Viperin suppresses T7 promoter dependent RNA synthesis-possible implications for its antiviral activity." Scientific Reports 8.1 (2018): 8100.
This study explores the antiviral activity of viperin, an interferon-inducible protein, focusing on its ability to inhibit RNA synthesis mediated by bacteriophage T7 polymerase in mammalian cells. The researchers demonstrated that viperin significantly reduces cytoplasmic RNA levels by specifically inhibiting T7 polymerase-dependent RNA synthesis without affecting RNA polymerase II-dependent transcription. This inhibition is shown to be specific to viperin, as other interferon-stimulated genes did not exhibit similar effects. The study also investigated the structural domains of viperin necessary for its inhibitory activity, finding that mutations in these domains abolished its function. This research provides insights into the broad-spectrum antiviral mechanisms of viperin and its potential role in inhibiting viral RNA synthesis across different species.
Creative Biolabs provided the anti-T7 polymerase antibody (Cat# MOB-0296MC) used in the Western blot analysis. This antibody was crucial for detecting T7 polymerase protein levels in various experimental setups, allowing the researchers to confirm that viperin does not affect the stability of T7 polymerase but specifically inhibits its RNA synthesis activity. By enabling accurate detection and analysis of T7 polymerase, Creative Biolabs' antibody supported the study's investigation into the mechanisms underlying viperin's antiviral functions.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
scFv Fragment Antibody
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-947-F(E) | Recombinant Anti-T7 RNA Polymerase Antibody Fab Fragment | RIA, IP, FuncS | Fab |
| MHH-947-F(E) | Recombinant Human Anti-T7 RNA Polymerase Antibody Fab Fragment | ELISA, WB, IF, FuncS | Fab |
Customer Reviews and Q&As
Customer Review
Q&As
-
Great for Polymerase Detection
The Anti-T7 RNA Polymerase Antibody has been fantastic for our polymerase detection assays. Its specificity and sensitivity are outstanding, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time. -
Reliable and Effective
We incorporated this antibody into our Western blot assays, and it has been a great addition. The specificity for T7 RNA polymerase is precise, consistently detecting the target protein with high clarity. The reliable performance has made it an essential tool in our research. -
Consistent High-Quality Results
This antibody has proven to be consistent and effective in our various assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for T7 RNA polymerase have been particularly beneficial.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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