Rabbit Anti-VPS4B Recombinant Antibody (clone DS3852AB)
CAT#: MOR-3852
Recombinant Rabbit anti-VPS4B monoclonal antibody for WB, FC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40393/1641_B10_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57649/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46445/103904_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46445/103904_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46445/103904_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46445/103904_A_4_6.jpg
Normal Tissue
Figure 7 Vagina
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46445/103904_B_1_2.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119541-VPS4B
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Specifications
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human VPS4B
- Species Reactivity
- Human
- Clone
- DS3852AB
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Reliable and Reproducible Results
In my experience, this antibody provides reliable and reproducible results, which is essential for validating our findings on VPS4B functions. Its consistency in performance across parallel experiments has made it a staple in our biochemical research.
Enhanced Experimental Outcomes
Utilizing the Anti-VPS4B antibody has enhanced our experimental outcomes dramatically. The high sensitivity ensures even low-abundance proteins are detected accurately, allowing us to delve deeper into the functions of VPS4B within various pathways.
Q&As
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How does this antibody specifically target VPS4B protein?
A: This antibody is designed to specifically recognize the VPS4B protein, a crucial component involved in membrane trafficking and the function of the endosomal sorting complex required for transport (ESCRT). Its specificity facilitates accurate detection and analysis in various research settings.
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Are there any conjugation options available for labeling this antibody?
A: The Anti-VPS4B Recombinant Antibody can be ordered with various conjugation options such as Biotin, PE, APC, FITC, and HRP. These conjugates provide versatility for multiple detection methods, enriching the utility of the antibody in different assay formats.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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