Mouse Anti-ssDNA Recombinant Antibody (clone BV04-01)

Polystyrene plates were coated with ssDNA and co-incubated with horseradish peroxidase-BV04-01 and the following inhibitors, mAb BV04-01 (triangles), BV04-01 Fab fragments (circles), and SCA 04-01 (squares).

Rumbley, C. A., Denzin, L. K., Yantz, L. S. Y. T., Tetin, S. Y., & Voss, E. W. (1993). Construction, characterization, and selected site-specific mutagenesis of an anti-single-stranded DNA single-chain autoantibody. Journal of Biological Chemistry, 268(18), 13667-13674.

Individual points represent mean values of triplicate trials with standard deviations (error bars). Points lacking error bars indicate standard deviations smaller than symbol. A, anti-ssDNA activity of fluorescein-purified BiSCA. Polystyrene wells were coated with ssDNA and incubated with BiSCA (10 μg/ml starting concentration) liganded with fluorescein. BiSCA binding to ssDNA was detected with 1A4 (non-ligand-inhibitable anti-4-4-20 antibody) (circles) or 1B7 (ligand-inhibitable anti-4-4-20 antibody) (1 μg/ml) (open triangles). BiSCA was compared to mAb 04-01 (crosses; solid line) and mAb 4-4-20 (closed triangles) (1 μg/ml starting concentration) detected with protein A-HRP. B, anti-ssDNA activity of ssDNA-purified BiSCA (triangles) as compared to equal molar concentrations (1 x 10⁻⁷ M) mAb 04-01 (crosses; solid line). mAb 4-4-20 was hibition of mAb 04-01 and BiSCA binding used as a negative control (circles). C, into ssDNA by soluble DNA. Singlestranded DNA-coated polystyrene wells were incubated with equal molar concentrations(1 x 10⁻⁷ M) of BiSCA (crosses; solid line) and mAb 04-01 (triangles) after preincubation overnight with calf thymus DNA (0.5 mg/ml starting concentration). Individual points represent mean values of duplicate trials.

Mallender, W. D., & Voss, E. W. (1994). Construction, expression, and activity of a bivalent bispecific single-chain antibody. Journal of Biological Chemistry, 269(1), 199-206.