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    PE-A*02:02/Human PSMA1 (FMECNLNEL) MHC Tetramer (CAT#: MHC-LC4279)

    This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of human PSMA1 of peptide FMECNLNEL covering 179-187 and A*02:02 molecule. The pMHC tetramer recognizes CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.

    Specific Inquiry
    • Size:
    Custom Production

    MHC tetramer custom production provides a vital tool for researchers seeking to understand and manipulate T-cell immunity with exceptional precision. Please specify your requirements, including peptide sequence, MHC allele, and desired fluorophore etc. We will respond as soon as possible with a tailored solution.

    Peptide Sequence *:
    Allele Requested *:
    Antigen Species:
    Antigen Molecule:
    Sequence Position:
    Form Requested *:
    Conjugation *:
    Size *:
    Comments:

    Disclaimer: Please note that the MHC reagents we offered have been produced with strict quality control (including gene sequencing, affinity purification, SDS-PAGE analysis, and verification of biotinylation), but many have not been confirmed to stain or activate specific T cells.

    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/if_selected.jpg
    Figure 2 IHC staining of human testis
    Figure 2 IHC staining of human testis
    (Immunohistochemical staining of human testis shows distinct cytoplasmic and nuclear positivity in cells in seminiferus ducts and Leydig cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/ihc_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Glial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuronal cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuropil
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/78642_B_7_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/78642_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Hepatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/78642_A_7_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Cells in tubules
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/78642_A_7_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Elongated or late spermatids
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/78642_A_5_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/78642_A_9_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000129084-PSMA1/subcellular

    Specifications

    • Allele
    • A*02:02
    • Class
    • Class I
    • MHC Species
    • Human
    • Antigen
    • PSMA1
    • Antigen Species
    • Human
    • Peptide
    • FMECNLNEL
    • Range
    • 179-187
    • Conjugate
    • PE
    • Application
    • FCM

    Target

    • Antigen Introduction
    • The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]
    • Alternative Names
    • PSMA1; Proteasome subunit alpha type-1; NU; HC2; PROS30; HEL-S-275

    Downloads

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