PE-H-2Kd/Influenza A virus HA (IYSTVASSL) MHC Tetramer
CAT#: MHC-LC532
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of Influenza-derived peptide of IYSTVASSL sequence covering 533-541 and H-2Kd molecule. The pMHC tetramer recognizes mouse CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.
Specifications
- Allele
- H-2Kd
- Class
- Class I
- MHC Species
- Mouse
- Antigen
- Influenza A virus HA
- Antigen Species
- Influenza A virus
- Peptide
- IYSTVASSL
- Range
- 533-541
- Conjugate
- PE
- Application
- FCM
Target
- Antigen Introduction
- Influenza A virus causes influenza in birds and some mammals, and is the only species of influenza virus A. Influenza virus A is a genus of the Orthomyxoviridae family of viruses. Strains of all subtypes of influenza A virus have been isolated from wild birds, although disease is uncommon. Some isolates of influenza A virus cause severe disease both in domestic poultry and, rarely, in humans. Occasionally, viruses are transmitted from wild aquatic birds to domestic poultry, and this may cause an outbreak or give rise to human influenza pandemics.
- Alternative Names
- Influenza A virus hemagglutinin; Influenza A virus HA
- Gene ID
- 3654620
- UniProt ID
- Q9Q0U6
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Exceptionally Specific
This MHC tetramer exceeded my expectations with its specificity. When applied in my influenza study, it consistently identified HA-specific CD8 T cells without cross-reactivity, simplifying my analysis process. The quality of the material was apparent from the robust signals obtained in flow cytometry.
Ease of Use in Flow Cytometry
The PE conjugate provides clear fluorescence, simplifying T cell analysis via flow cytometry. We've found it user-friendly and effective for generating reproducible data.
Strong Performance in Viral Studies
We've incorporated this tetramer into our viral pathogenesis studies. Its targeted approach to T cell detection contributes to our understanding of immune responses in viral infections.
Q&As
-
What is the primary application of this MHC tetramer?
A: The PE-H-2Kd/Influenza A virus HA MHC Tetramer is primarily used for the analysis of individual antigen-specific CD8 T cells in research settings. It aids in monitoring immune responses, particularly in viral studies like those involving influenza.
-
Can this tetramer be used for any other applications besides FCM?
A: While flow cytometry (FCM) is the primary application, the tetramer may also support other assays that require the identification or quantification of antigen-specific T cells, provided the experimental setup is adapted accordingly.
-
What is the significance of the PE conjugation in this tetramer?
A: The PE (phycoerythrin) conjugation enables the tetramer to be visualized and quantified via flow cytometry, providing a brightly fluorescent marker for antigen-specific T cells.
View the frequently asked questions answered by Creative Biolabs Support.
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