Human Anti-Human VEGF Recombinant Antibody; Fab Fragment (TP-104CL) (CAT#: TP-104CL)
This product is a recombinant humanized IgG1 kappa isotype monoclonal antibody fragment designed for intraocular use. The antibody binds to and inhibits the biologic activity of human vascular endothelial growth factor A (VEGF-A). It has a molecular weight of approximately 48 kilodaltons and is produced by an E. coli expression system in a nutrient medium containing the antibiotic tetracycline (tetracycline is not detectable in the final product).
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Anti-Human VEGF Antibody Fab Fragment (TP-104CL) in WB
Western blot analysis of TP-104CL was performed with Recombinant Human VEGF protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TP-104CL and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 2 Anti-Human VEGF Antibody Fab Fragment (TP-104CL) in ELISA
ELISA analysis of TP-104CL was performed by coating with Recombinant Human VEGF Protein. Then blocked with BSA, and incubated with Anti-VEGF Therapeutic Antibody (ranibizumab) (TP-104CL). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-Human VEGF Antibody Fab Fragment (TP-104CL) in DB
Antigen: Human VEGF protein
Antibody incubation concentration: 2ng/μL.
Specifications
- Type
- Biotech
Target
- Function
- Humanized mAb fragment that binds isoforms of vascular endothelial growth factor A (VEGFA)
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Customer Reviews and Q&As
Excellent for VEGF Studies
Essential for Angiogenesis Research
Valuable Research Asset
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TP-104CL, RRID: AB_3112056)
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For Research Use Only. Not For Clinical Use.
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