Mouse Anti-ARHGAP35 Recombinant Antibody (clone 28B10)
CAT#: MOB-3897z
This product is a mouse antibody that recognizes human ARHGAP35. It can be used in the following applications: WB, IP, IF, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & aggresome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56470/970_D8_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55184/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56470/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55184/136657_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55184/136657_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55184/136657_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55184/136657_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55184/136657_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160007-ARHGAP35
❮
❯
Specifications
- Immunogen
- Human Rho GTPase Activating Protein 35
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Antibody Isotype
- IgG1, κ
- Specificity
- Human ARHGAP35
- Species Reactivity
- Human
- Clone
- 28B10
- Applications
- WB, IP, IF, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Immunoprecipitation Assay, Immunofluorescence Assay, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- ARHGAP35; Rho GTPase-Activating Protein 35; GRF-1; Glucocorticoid Receptor DNA Binding Factor 1; GRF1; P190ARhoGAP; KIAA1722; Glucocorticoid Receptor Repression Factor 1; GRLF1; P190A; Glucocorticoid Receptor DNA-Binding Factor 1; P190-A; Rho GTPase Activ
- UniProt ID
- Q9NRY4
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for MOB-3897z. Click the button above to contact us or submit your feedback about this product.
High Specificity In Western Blot
The clone 28B10 performed exceptionally well in our Western Blot experiments targeting ARHGAP35. We detected a clear band corresponding to the expected molecular weight without non-specific binding. It is refreshing to use a recombinant mouse antibody that offers such high specificity for the human target.
Successful Immunoprecipitation Results
We incorporated this antibody into our immunoprecipitation (IP) protocols to study the glucocorticoid receptor DNA binding factor. The pull-down efficiency was high, and subsequent analysis confirmed the presence of the target protein. This product has become a standard reagent in our lab for studying Rho GTPase pathways.
Q&As
-
Which applications are recommended for this mouse anti-ARHGAP35 antibody?
A: This recombinant mouse antibody (clone 28B10) has been reported for use in Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), and Enzyme-linked Immunosorbent Assay (ELISA). It is a versatile reagent for detecting Human ARHGAP35 in various experimental setups.
-
Is this antibody monoclonal, and what is the isotype?
A: Yes, this is a recombinant mouse monoclonal antibody, clone 28B10. The isotype is Mouse IgG1, kappa. It is produced to ensure high purity (>95% as determined by SDS-PAGE) and specific recognition of the Human Rho GTPase Activating Protein 35.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ARHGAP35"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2440MZ | Mouse Anti-ARHGAP35 Recombinant Antibody (clone 3490E4b) | WB | Mouse IgG1 |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, IP, IF, FuncS, FC, Neut, ELISA
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
-2.png)
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, ELISA, FuncS
Application: WB, IF, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
