Recombinant Human Anti-Streptococcus pneumoniae 23F Antibody Fab Fragment (023.102)
CAT#: PABX-173-F (E)
Anti-Streptococcus pneumoniae 23F antibody is a Human of Fab class that binds to an Streptococcus pneumoniae 23F.
Published Data
-1.png)
Figure 1 Structures of Abs 023.102, pn132p2C05, and KE5 with bound ligands.
The methylated core disaccharide RGP bound to the paratope of Ab 023.102.
Bryson, S., Thomson, C. A., Risnes, L. F., Dasgupta, S., Smith, K., Schrader, J. W., & Pai, E. F. (2016). Structures of Preferred Human IgV Genes–Based Protective Antibodies Identify How Conserved Residues Contact Diverse Antigens and Assign Source of Specificity to CDR3 Loop Variation. The Journal of Immunology, 196(11), 4723-4730.
-2.png)
Figure 2 Germline V-gene–encoded Igs derived from the hypermutated anti-23F Ab (023.102) bind specifically to the 23F polysaccharide in ELISA; importance of the CDRH3 and CDRL3.
A, Left panel, Binding at high concentrations of S.sJ1, which uses the germline sequences of IGHV3-30, IGHJ4, IGKV3-11, and IGKJ1, but with the CDRH3 seen in the hypermutated anti-23F Ab (023.102). Note that binding is observed at far lower concentrations by S.sJ1Ala with a single mutation of Trp to Ala in 96(L) of CDRL3. Right panel, Binding at high concentrations of S.mJ5 using the L chain of the germline Vgene-encoded ancestor of an anti–AD-2S1 Ab and the germline IGKJ5 with a small hydrophilic residue at 96(L) but lacking the additional junctional residue Asp95b(L), whereas, S.sJ5Gly, which has the additional Asp95b(L) binds at lower concentrations to the 23F PPS. No binding of M2.sJ1Ala is observed that has the M2 H chain with the CDRH3 of the germline V-gene–encoded anti–AD-2S1 Abs. Data are the mean 6 SD of triplicate measurements. B, Failure of the germline V-gene–encoded Abs S.sJ1, S.sJ1Ala, S.sJ5Gly, S.mJ5, or M2.sJ1Ala to bind to the 14 PPS (left panel) or 6B PPS (right panel). C, Failure of the germline V-gene–encoded Abs S.sJ1, S.sJ1Ala, S.sJ5Gly, S.mJ5, and M2.sJ1Ala to bind the AD-2S1 peptide. KE5, the hypermutated anti-AD-2S1 mAb, is shown as a positive control. D, A mouse IgM mAb against the human IgG CH3 domain, which forms complexes with S.sJ1 and S.sJ1Ala, increases their avidity for 23F in ELISA. There was no binding observed by the anti–AD-2S1 Ab KE5, either alone or complexed with the IgM. Binding also was not observed with IgM alone (data not shown).
Thomson, C. A., Little, K. Q., Reason, D. C., & Schrader, J. W. (2011). Somatic diversity in CDR3 loops allows single V-genes to encode innate immunological memories for multiple pathogens. The Journal of Immunology, 186(4), 2291-2298.
❮
-1.webp)
-2.webp)
❯
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- Fab
- Specificity
- Tested positive against native Streptococcus pneumoniae 23F
- Species Reactivity
- Streptococcus pneumoniae
- Clone
- 023. 102
- Applications
- ELISA 1:10000-1:20000
Neut (assay dependent)
FuncS (assay dependent)
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- 23F strep; Streptococcus pneumoniae type 23F
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for PABX-173-F (E). Click the button above to contact us or submit your feedback about this product.
Excellent binding efficiency
I've been using the 023.102 Fab fragment for my research on pneumococcal infections, and it's surpassed my expectations. Its high specificity and excellent binding efficiency have made our ELISA assays highly reliable. The fragment's smaller size has improved our in vivo studies, allowing for better tissue penetration and accurate localization. Highly recommended!
Good specificity for the 23F serotype
This Fab fragment has significantly enhanced the accuracy of our pathogen detection assays. Its specificity for the 23F serotype has reduced cross-reactivity issues we faced with other antibodies. The ease of storage and stability make it a convenient choice for routine use.
Great for ELISA
The 023.102 Fab fragment has exceeded our expectations in sensitivity and specificity in detecting the 23F serotype. We utilized it in ELISA with excellent outcomes. The product's overall reliability has streamlined our workflow and improved our experimental accuracy.
Q&As
-
How does the Recombinant Human Anti-Streptococcus pneumoniae 23F Antibody Fab Fragment (023.102) compare with whole antibodies in terms of binding efficiency?
A: The Fab fragment 023.102 retains comparable binding efficiency to the whole antibody but offers better tissue penetration due to its smaller size. Its specificity to the 23F serotype ensures it targets Streptococcus pneumoniae effectively, without cross-reactivity seen in whole antibodies.
-
Under which recommended conditions should the Recombinant Human Anti-Streptococcus pneumoniae 23F Antibody Fab Fragment (023.102) be stored to ensure its stability?
A: The Fab fragment should be stored at -80°C for long-term stability. For short-term use, storing at 4°C is acceptable, but repeated freeze-thaw cycles should be avoided to prevent degradation or loss of binding activity.
-
What is the recommended dosage of Fab fragment 023.102 for use in an ELISA assay?
A: The ideal concentration for ELISA varies, although a dilution range of 1-10 µg/mL is recommended. Titration assays should be used to establish the optimal concentration for their specific use, guaranteeing maximal binding with minimal background noise.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABX-173-F (E), RRID: AB_3111704)
Copy citation
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 023. 102"
- CAT
- Product Name
See other products for "23F strep"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-001 | Recombinant Human Anti-23F strep Antibody scFv Fragment (C05) | WB, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-001 | Recombinant Human Anti-23F strep Antibody Fab Fragment (C05) | WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-173 | Recombinant Human Anti-Streptococcus pneumoniae 23F Antibody (023.102) | Neut, ELISA, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-173-S (P) | Recombinant Human Anti-Streptococcus pneumoniae 23F Antibody scFv Fragment (023.102) | Neut, ELISA, FuncS | scFv |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: WB, IF, FuncS
Application: ELISA, FuncS, IHC, IF, FC, ADCC
Application: ELISA
-5.jpg)
Application: FC, IHC-Fr, IP, ELISA, Block
-2.png)
-4.jpg)
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
