Mouse Anti-ανβ8 Recombinant Antibody; Fab Fragment (HPAB-N0303-YC-F(E)) (CAT#: HPAB-N0303-YC-F(E))

This product is a recombinant Mouse antibody Fab fragment that recognizes ανβ8. The antibody was purified by affinity chromatography.


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SDS-PAGE

Figure 1 Mouse Anti-ανβ8 Recombinant Antibody; Fab Fragment (HPAB-N0303-YC-F(E)) in SDS-PAGE

Figure 1 Mouse Anti-ανβ8 Recombinant Antibody; Fab Fragment (HPAB-N0303-YC-F(E)) in SDS-PAGE

SDS-PAGE analysis of HPAB-N0303-YC-F(E) in non-reduced (Lane 2, 1.5 μg) and β-mercaptoethanol-reduced (Lane 1, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 20-25 kDa.

DB

Figure 2 Mouse Anti-ανβ8 Recombinant Antibody; Fab Fragment (HPAB-N0303-YC-F(E)) in DB

Figure 2 Mouse Anti-ανβ8 Recombinant Antibody; Fab Fragment (HPAB-N0303-YC-F(E)) in DB

Dot Blot analysis of HPAB-N0303-YC-F(E) was performed by coating with Recombinant Human Integrin alpha V beta 8 Protein (His tag).

HPAB-N0303-YC-F(E) incubation concentration: 2 μg/mL.
HRP conjugated Anti-His tag as a secondary antibody (1: 2000)

ELISA

Figure 3 Mouse Anti-ανβ8 Recombinant Antibody; Fab Fragment (HPAB-N0303-YC-F(E)) in ELISA

Figure 3 Mouse Anti-ανβ8 Recombinant Antibody; Fab Fragment (HPAB-N0303-YC-F(E)) in ELISA

ELISA analysis of HPAB-N0303-YC-F(E) was performed by coating with Recombinant Human Integrin alpha V beta 8 Protein (His tag). Then blocked with BSA and incubated with HPAB-N0303-YC-F(E). The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Immunogen
  • Recombinant human Integrin alpha V beta 8 (ανβ8) protein
  • Host Species
  • Mouse
  • Type
  • Mouse Fab
  • Specificity
  • Human ανβ8
  • Species Reactivity
  • Human, Mouse
  • Applications
  • ELISA, Block

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Integrin ανβ8; β8

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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