This product is a recombinant Mouse antibody Fab fragment that recognizes ανβ8. The antibody was purified by affinity chromatography.
Figure 1 Mouse Anti-ανβ8 Recombinant Antibody; Fab Fragment (HPAB-N0303-YC-F(E)) in SDS-PAGE
SDS-PAGE analysis of HPAB-N0303-YC-F(E) in non-reduced (Lane 2, 1.5 μg) and β-mercaptoethanol-reduced (Lane 1, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 20-25 kDa.
Figure 2 Mouse Anti-ανβ8 Recombinant Antibody; Fab Fragment (HPAB-N0303-YC-F(E)) in DB
Dot Blot analysis of HPAB-N0303-YC-F(E) was performed by coating with Recombinant Human Integrin alpha V beta 8 Protein (His tag).
HPAB-N0303-YC-F(E) incubation concentration: 2 μg/mL.
HRP conjugated Anti-His tag as a secondary antibody (1: 2000)
Figure 3 Mouse Anti-ανβ8 Recombinant Antibody; Fab Fragment (HPAB-N0303-YC-F(E)) in ELISA
ELISA analysis of HPAB-N0303-YC-F(E) was performed by coating with Recombinant Human Integrin alpha V beta 8 Protein (His tag). Then blocked with BSA and incubated with HPAB-N0303-YC-F(E). The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-N0303-YC | Mouse Anti-ανβ8 Recombinant Antibody (HPAB-N0303-YC) | ELISA, Block | Mouse IgG2a |
HPAB-N0304-YC | Human Anti-ανβ8 Recombinant Antibody (HPAB-N0304-YC) | ELISA, Block | Humanized IgG |
HPAB-N0305-YC | Human Anti-ανβ8 Recombinant Antibody (HPAB-N0305-YC) | ELISA, Block | Humanized IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-N0303-YC-S(P) | Mouse Anti-ανβ8 Recombinant Antibody; scFv Fragment (HPAB-N0303-YC-S(P)) | ELISA, Block | Mouse scFv |
HPAB-N0304-YC-S(P) | Human Anti-ανβ8 Recombinant Antibody; scFv Fragment (HPAB-N0304-YC-S(P)) | ELISA, Block | Human scFv |
HPAB-N0305-YC-S(P) | Human Anti-ανβ8 Recombinant Antibody; scFv Fragment (HPAB-N0305-YC-S(P)) | ELISA, Block | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-N0304-YC-F(E) | Human Anti-ανβ8 Recombinant Antibody; Fab Fragment (HPAB-N0304-YC-F(E)) | ELISA, Block | Humanized Fab |
HPAB-N0305-YC-F(E) | Human Anti-ανβ8 Recombinant Antibody; Fab Fragment (HPAB-N0305-YC-F(E)) | ELISA, Block | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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